BRENDA - Enzyme Database

CDP-2,3-di-O-geranylgeranyl-sn-glycerol:L-serine O-archaetidyltransferase (archaetidylserine synthase) in the methanogenic archaeon Methanothermobacter thermautotrophicus

Morii, H.; Koga, Y.; J. Bacteriol. 185, 1181-1189 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.38
Triton X-100
activity is dependent on TRiton X-100
Methanothermobacter thermautotrophicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.38
Mn2+
stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective
Methanothermobacter thermautotrophicus
2.7.8.38
additional information
the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Bacillus subtilis
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.67
Methanothermobacter thermautotrophicus
-
-
-
2.7.7.67
Methanothermobacter thermautotrophicus DSM 1053
-
-
-
2.7.8.8
Escherichia coli
-
-
-
2.7.8.38
Bacillus subtilis
-
-
-
2.7.8.38
Methanothermobacter thermautotrophicus
O27106
-
-
2.7.8.38
Methanothermobacter thermautotrophicus DSM 1053
O27106
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.8.38
CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol + L-serine = CMP + 2,3-bis-(O-geranylgeranyl)-sn-glycero-1-phospho-L-serine
(1)
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
692823
Methanothermobacter thermautotrophicus
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
692823
Methanothermobacter thermautotrophicus DSM 1053
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.8.8
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
41% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Escherichia coli
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.8
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Escherichia coli
CMP + 1,2-diacyl-sn-glycerol-3-phospho-L-serine
-
-
-
?
2.7.8.8
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
17% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Escherichia coli
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.8
additional information
very low activity with (less than 10% compared to CDP-1,2-diacylglycerol with fatty acids from lecithin): CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol, CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol, CDP-2,3-bis-O-(phytanyl)-sn-glycerol
692823
Escherichia coli
?
-
-
-
-
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-dioleoyl-sn-glycerol + L-serine
143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.38
60
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.8.38
50
70
50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity
Methanothermobacter thermautotrophicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.38
8.5
-
assay at
Methanothermobacter thermautotrophicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.38
Triton X-100
activity is dependent on TRiton X-100
Methanothermobacter thermautotrophicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.38
Mn2+
stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective
Methanothermobacter thermautotrophicus
2.7.8.38
additional information
the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Bacillus subtilis
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
692823
Methanothermobacter thermautotrophicus
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
692823
Methanothermobacter thermautotrophicus DSM 1053
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.7.67
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
-
-
-
?
2.7.8.8
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
41% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Escherichia coli
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.8
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Escherichia coli
CMP + 1,2-diacyl-sn-glycerol-3-phospho-L-serine
-
-
-
?
2.7.8.8
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
17% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Escherichia coli
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.8
additional information
very low activity with (less than 10% compared to CDP-1,2-diacylglycerol with fatty acids from lecithin): CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol, CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol, CDP-2,3-bis-O-(phytanyl)-sn-glycerol
692823
Escherichia coli
?
-
-
-
-
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
2.7.8.38
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
2.7.8.38
CDP-2,3-dioleoyl-sn-glycerol + L-serine
143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.38
60
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.8.38
50
70
50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.38
8.5
-
assay at
Methanothermobacter thermautotrophicus
General Information
EC Number
General Information
Commentary
Organism
2.7.7.67
metabolism
the enzyme is involved in the biosynthesis of polar lipids in archaea
Methanothermobacter thermautotrophicus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.7.67
metabolism
the enzyme is involved in the biosynthesis of polar lipids in archaea
Methanothermobacter thermautotrophicus