Literature summary extracted from

Morii, H.; Koga, Y.
CDP-2,3-di-O-geranylgeranyl-sn-glycerol:L-serine O-archaetidyltransferase (archaetidylserine synthase) in the methanogenic archaeon Methanothermobacter thermautotrophicus (2003), J. Bacteriol., 185, 1181-1189.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.8.38	Triton X-100	activity is dependent on TRiton X-100	Methanothermobacter thermautotrophicus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.8.38	Mn2+	stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective	Methanothermobacter thermautotrophicus
2.7.8.38	additional information	the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus	Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	Methanothermobacter thermautotrophicus	the enzyme is involved in the biosynthesis of polar lipids in archaea	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	Methanothermobacter thermautotrophicus DSM 1053	the enzyme is involved in the biosynthesis of polar lipids in archaea	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	Methanothermobacter thermautotrophicus	the enzyme is involved in the biosynthesis of polar lipids in archaea	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	Methanothermobacter thermautotrophicus DSM 1053	the enzyme is involved in the biosynthesis of polar lipids in archaea	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine	Bacillus subtilis	120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine	-	?
2.7.8.38	CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine	Methanothermobacter thermautotrophicus	86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.67	Methanothermobacter thermautotrophicus	-	-	-
2.7.7.67	Methanothermobacter thermautotrophicus DSM 1053	-	-	-
2.7.8.8	Escherichia coli	-	-	-
2.7.8.38	Bacillus subtilis	-	-	-
2.7.8.38	Methanothermobacter thermautotrophicus	O27106	-	-
2.7.8.38	Methanothermobacter thermautotrophicus DSM 1053	O27106	-	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.7.8.38	CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol + L-serine = CMP + 2,3-bis-(O-geranylgeranyl)-sn-glycero-1-phospho-L-serine	(1)	Methanothermobacter thermautotrophicus	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	-	Methanothermobacter thermautotrophicus	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	the enzyme is involved in the biosynthesis of polar lipids in archaea	Methanothermobacter thermautotrophicus	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	-	Methanothermobacter thermautotrophicus DSM 1053	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.7.67	CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate	the enzyme is involved in the biosynthesis of polar lipids in archaea	Methanothermobacter thermautotrophicus DSM 1053	diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol	-	?
2.7.8.8	CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine	41% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Escherichia coli	CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine	-	?
2.7.8.8	CDP-1,2-diacylglycerol + L-serine	fatty acids from lecithin	Escherichia coli	CMP + 1,2-diacyl-sn-glycerol-3-phospho-L-serine	-	?
2.7.8.8	CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine	17% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Escherichia coli	CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine	-	?
2.7.8.8	additional information	very low activity with (less than 10% compared to CDP-1,2-diacylglycerol with fatty acids from lecithin): CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol, CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol, CDP-2,3-bis-O-(phytanyl)-sn-glycerol	Escherichia coli	?	-	?
2.7.8.38	CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Bacillus subtilis	CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus	CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine	-	?
2.7.8.38	CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus DSM 1053	CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine	-	?
2.7.8.38	CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine	102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Bacillus subtilis	CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine	64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus	CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine	64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus DSM 1053	CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-diacylglycerol + L-serine	fatty acids from lecithin	Bacillus subtilis	CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-diacylglycerol + L-serine	fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus	CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-1,2-diacylglycerol + L-serine	fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus DSM 1053	CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine	-	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	the enzyme is involved in the biosynthesis of polar lipids in archaea	Methanothermobacter thermautotrophicus	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Bacillus subtilis	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase	Methanothermobacter thermautotrophicus	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	the enzyme is involved in the biosynthesis of polar lipids in archaea	Methanothermobacter thermautotrophicus DSM 1053	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine	i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase	Methanothermobacter thermautotrophicus DSM 1053	CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine	i.e. unsaturated archaetidylserine	?
2.7.8.38	CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine	280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus	CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine	-	?
2.7.8.38	CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine	120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Bacillus subtilis	CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine	-	?
2.7.8.38	CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine	86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate	Methanothermobacter thermautotrophicus	CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine	-	?
2.7.8.38	CDP-2,3-dioleoyl-sn-glycerol + L-serine	143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin	Bacillus subtilis	CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol:L-serine O-archaetidyltransferase	-	Bacillus subtilis
2.7.8.38	CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol:L-serine O-archaetidyltransferase	-	Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.8.38	60	-	-	Methanothermobacter thermautotrophicus

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.7.8.38	50	70	50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity	Methanothermobacter thermautotrophicus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7.8.38	8.5	-	assay at	Methanothermobacter thermautotrophicus

General Information

EC Number	General Information	Comment	Organism
2.7.7.67	metabolism	the enzyme is involved in the biosynthesis of polar lipids in archaea	Methanothermobacter thermautotrophicus