Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Addlesee, H.A.; Hunter, C.N.
    Physical mapping and functional assignment of the geranylgeranyl-bacteriochlorophyll reductase gene, bchP, of Rhodobacter sphaeroides (1999), J. Bacteriol., 181, 7248-7255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.111 expression in Escherichia coli Cereibacter sphaeroides
1.3.1.111 gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5 Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.111 additional information a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+ Cereibacter sphaeroides the enzyme is involved in phythylation of bacteriochlorophyll a phytyl-bacteriochlorophyll a + 3 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.111 Cereibacter sphaeroides Q9Z5D4
-
-
1.3.1.111 Cereibacter sphaeroides Q9Z5D4 gene bchP
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+ activity assay is performed in the dark Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
 Cereibacter sphaeroides phytyl-bacteriochlorophyll a + 3 NADP+
-
 ?
1.3.1.111 geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+ the enzyme is involved in phythylation of bacteriochlorophyll a Cereibacter sphaeroides phytyl-bacteriochlorophyll a + 3 NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.111 BchP
-
 Cereibacter sphaeroides
1.3.1.111 geranylgeranyl-bacteriochlorophyll reductase
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.111 30
-
 assay at Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.111 8
-
 assay at Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.111 NADP+
-
 Cereibacter sphaeroides
1.3.1.111 NADPH
-
 Cereibacter sphaeroides

General Information

EC Number General Information Comment Organism
1.3.1.111 malfunction a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol Cereibacter sphaeroides
1.3.1.111 metabolism the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis, pathway for the phytylation of bacteriochlorophyll a, overview Cereibacter sphaeroides
1.3.1.111 physiological function the bacteriochlorophyll of the purple photosynthetic bacterium Rhodobacter sphaeroides is esterified with phytol. The presence of this alcohol moiety is essential for the correct assembly of the photosynthetic apparatus. The enzyme is essential and responsible for the reduction of the alcohol moiety of (bacterio)chlorophyll to phytol Cereibacter sphaeroides