EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.3.1 | D99A | secondary kinetic isotope effects similar to wild-type. Mutation does not significantly decrease the contribution of coupled motion/hydrogen tunneling to the enzymatic reaction | Comamonas testosteroni |
5.3.3.1 | Y14F | secondary kinetic isotope effects similar to wild-type. Mutation does not significantly decrease the contribution of coupled motion/hydrogen tunneling to the enzymatic reaction | Comamonas testosteroni |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.3.1 | Comamonas testosteroni | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.3.3.1 | a 3-oxo-DELTA5-steroid = a 3-oxo-DELTA4-steroid | enzyme enhances the coupled motion/hydrogen tunneling contribution to the rate acceleration over the solution reaction | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.1 | androst-5-ene-3,17-dione | alpha-secondary deuterium kinetic isotope effects at C6 of the steroid. Presence of coupled motion/hydrogen tunneling in the enzyme-catalyzed reaction | Comamonas testosteroni | androst-4-ene-3,17-dione | - |
r |