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Literature summary extracted from

  • Bennett, M.T.; Rodgers, M.T.; Hebert, A.S.; Ruslander, L.E.; Eisele, L.; Drohat, A.C.
    Specificity of human thymine DNA glycosylase depends on N-glycosidic bond stability (2006), J. Am. Chem. Soc., 128, 12510-12519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.29 expression in Escherichia coli Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.29 Homo sapiens Q13569
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.29
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.29 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-bromocytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-bromouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-chlorouracil-mismatched double-stranded DNA + H2O hTDG removes 5-chlorouracil 572fold faster than thymine Homo sapiens 5-chlorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-fluorocytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-fluorocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-fluorouracil-mismatched double-stranded DNA + H2O hTDG removes 5-fluorouracil 78fold faster than uracil Homo sapiens 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-hydroxcytosine-mismatched double-stranded DNA + H2O hTDG readily excises cytosine analogues with improved leaving ability, including 5-fluorocytosine, 5-bromocytosine, and 5-hydroxycytosine, indicating that cytosine has access to the active site. hTDG specificity depends on N-glycosidic bond stability, and the discrimination against cytosine is due largely to its very poor leaving ability rather than its exclusion from the active site Homo sapiens 5-hydroxycytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O cleaves thymine from mutagenic G/T mispairs. Recognizes many additional lesions, and has a strong preference for nucleobases paired with guanine rather than adenine. hTDG avoids cytosine, despite the million-fold excess of normal G/C pairs over G/T mispairs Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.29 hTDG
-
 Homo sapiens
3.2.2.29 thymine DNA glycosylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.29 0.0036
-
 thymine-mismatched double-stranded DNA cleavage of thymine from G/T mismatch Homo sapiens
3.2.2.29 0.043
-
 uracil-mismatched double-stranded DNA cleavage of uracil from G/U mismatch Homo sapiens
3.2.2.29 2.1
-
 5-chlorouracil-mismatched double-stranded DNA cleavage of 5-chlorouracil from G/5-chlorouracil mismatch Homo sapiens
3.2.2.29 3.7
-
 5-fluorouracil-mismatched double-stranded DNA cleavage of 5-fluorouracil from G/5-fluorouracil mismatch Homo sapiens