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Literature summary extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.
    Unfolding and refolding of Aspergillus niger PhyB phytase: role of disulfide bridges (2008), J. Agric. Food Chem., 56, 8179-8183.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.26 the role of disulfide bridges in the folding of Aspergillus niger PhyB is investigated using dynamic light scattering. Guanidinium chloride (1 M) unfolds phytase. Its removal by dialysis refolds the protein. Tris(2-carboxyethyl)phosphine (TCEP) reduces the refolding activity by 68%. The hydrodynamic radius of PhyB phytase decreases from 5.5 to 4.14 nm when the protein is subjected to 1.0 M GuCl concentration. The active homodimer, is reduced to a monomer Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 183000
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homodimer, dynamic light scattering Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Aspergillus niger
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the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Aspergillus niger ? + phosphate
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?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 homodimer dynamic light scattering, 2*92000 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 PhyB
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Aspergillus niger
3.1.3.26 phytase
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Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 58
-
assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 2.5
-
-
Aspergillus niger