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Literature summary extracted from

  • Landete, J.M.; Rodriguez, H.; de Las Rivas, B.; Munoz, R.
    Characterization of a benzyl alcohol dehydrogenase from Lactobacillus plantarum WCFS1 (2008), J. Agric. Food Chem., 56, 4497-4503.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.90 gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109 Lactiplantibacillus plantarum WCFS1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 2-mercaptoethanol 10 mM, 76% inhibition Lactiplantibacillus plantarum
1.1.1.90 2-mercaptoethanol inhibits 76% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 Ag+ inhibits 58% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 AgNO3 10 mM, 58% inhibition Lactiplantibacillus plantarum
1.1.1.90 DMSO 10 mM, 20% inhibition Lactiplantibacillus plantarum
1.1.1.90 DMSO inhibits 20% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 DTT 10 mM, 84% inhibition Lactiplantibacillus plantarum
1.1.1.90 DTT inhibits 84% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 EDTA 10 mM, 22% inhibition Lactiplantibacillus plantarum
1.1.1.90 EDTA inhibits 22% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 SDS 10 mM, 86% inhibition Lactiplantibacillus plantarum
1.1.1.90 SDS inhibits 86% at 10 mM Lactiplantibacillus plantarum WCFS1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.23
-
 benzyl alcohol
-
 Lactiplantibacillus plantarum
1.1.1.90 0.23
-
 benzyl alcohol pH 5.0, 30°C Lactiplantibacillus plantarum WCFS1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.90 CaCl2 10 mM, 1.3fold increase in activity Lactiplantibacillus plantarum
1.1.1.90 CaCl2 increases the enzyme activity by 27% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 CdCl2 10 mM, 1.3fold increase in activity Lactiplantibacillus plantarum
1.1.1.90 CdCl2 increases the enzyme activity by 25% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 KBr 10 mM, 1.1fold increase in activity Lactiplantibacillus plantarum
1.1.1.90 KBr increases the enzyme activity by 11% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 MgCl2 10 mM, 1.2fold increase in activity Lactiplantibacillus plantarum
1.1.1.90 MgCl2 increases the enzyme activity by 22% at 10 mM Lactiplantibacillus plantarum WCFS1
1.1.1.90 additional information no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80 Lactiplantibacillus plantarum WCFS1
1.1.1.90 Zn zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved Lactiplantibacillus plantarum
1.1.1.90 Zn2+ dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved Lactiplantibacillus plantarum WCFS1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 40000
-
 x * 40000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum WCFS1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 benzaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 benzyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
 benzaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 coniferyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
 coniferyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 geraniol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 geranial + NAD(P)H + H+
-
 ?
1.1.1.90 nerol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
 ?
1.1.1.90 phenethyl alcohol + NAD(P)+ Lactiplantibacillus plantarum WCFS1
-
 phenylacetaldehyde + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Lactiplantibacillus plantarum
-
-
-
1.1.1.90 Lactiplantibacillus plantarum WCFS1
-
 gene lp_3054
-
1.1.1.90 Lactiplantibacillus plantarum WCFS1 NCIMB 8826
-
 gene lp_3054
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90 recombinant lp_3054 from Escherichia coli strain JM109 Lactiplantibacillus plantarum WCFS1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 benzaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 benzyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 NCIMB 8826 benzaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 benzyl alcohol + NAD+ less active in the presence of NADP+ than in the presence of NAD+ Lactiplantibacillus plantarum benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NADP+ less active in the presence of NADP+ than in the presence of NAD+ Lactiplantibacillus plantarum benzaldehyde + NADPH
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+ 67% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 NCIMB 8826 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD(P)+ 67% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 NCIMB 8826 cinnamyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 cinnamyl alcohol + NAD+ 67% of the activity with benzyl alcohol Lactiplantibacillus plantarum cinnamyl aldehyde + NADH + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 coniferyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD(P)+ 59% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 coniferyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 NCIMB 8826 coniferyl aldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 coniferyl alcohol + NAD+ 59% of the activity with benzyl alcohol Lactiplantibacillus plantarum coniferyl aldehyde + NADH + H+
-
 ?
1.1.1.90 geraniol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 geranial + NAD(P)H + H+
-
 ?
1.1.1.90 geraniol + NAD(P)+ 105% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 geranial + NAD(P)H + H+
-
 ?
1.1.1.90 geraniol + NAD+ 105% of the activity with benzyl alcohol Lactiplantibacillus plantarum (2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
 ?
1.1.1.90 additional information no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid Lactiplantibacillus plantarum WCFS1 ?
-
 ?
1.1.1.90 additional information no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid Lactiplantibacillus plantarum WCFS1 NCIMB 8826 ?
-
 ?
1.1.1.90 nerol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
 ?
1.1.1.90 nerol + NAD(P)+ 116% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
 ?
1.1.1.90 nerol + NAD+ 116% of the activity with benzyl alcohol Lactiplantibacillus plantarum (2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
 ?
1.1.1.90 phenethyl alcohol + NAD(P)+
-
 Lactiplantibacillus plantarum WCFS1 phenylacetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 phenethyl alcohol + NAD(P)+ 73% activity compared to activity with benzyl alcohol Lactiplantibacillus plantarum WCFS1 phenylacetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.90 phenethyl alcohol + NAD+ 73% of the activity with benzyl alcohol Lactiplantibacillus plantarum phenylacetaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.90 ? x * 40000, recombinant enzyme, SDS-PAGE Lactiplantibacillus plantarum WCFS1

Synonyms

EC Number Synonyms Comment Organism
1.1.1.90 BADH
-
 Lactiplantibacillus plantarum WCFS1
1.1.1.90 benzyl alcohol dehydrogenase
-
 Lactiplantibacillus plantarum WCFS1
1.1.1.90 More the enzyme is a member of the family of NAD(P)-dependent long-chain zinc-dependent alcohol dehydrogenases Lactiplantibacillus plantarum WCFS1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.90 30
-
-
 Lactiplantibacillus plantarum
1.1.1.90 30
-
-
 Lactiplantibacillus plantarum WCFS1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.90 20 50 20°C: about 60% of maximal activity, 50°C: about 70% of maximal activity Lactiplantibacillus plantarum
1.1.1.90 25 50 activity range Lactiplantibacillus plantarum WCFS1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.90 30 50 the purified recombinant enzyme remains quite stable Lactiplantibacillus plantarum WCFS1
1.1.1.90 70
-
 purified recombinant enzyme, BADH activity significantly decreases to 16% of maximal activity Lactiplantibacillus plantarum WCFS1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 3.4
-
 benzyl alcohol pH 5.0, 30°C Lactiplantibacillus plantarum WCFS1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 5
-
-
 Lactiplantibacillus plantarum
1.1.1.90 5
-
-
 Lactiplantibacillus plantarum WCFS1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.90 3.5 6 pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity Lactiplantibacillus plantarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+ preferred cofactor compared to NADP+, all of the residues involved in cofactor binding are conserved. Residues Thr178 and Val203 interact with the nicotinamide ring, polypeptide backbone nitrogen atoms of residues Gly201 and Gly202 interact with an oxygen of the phosphate proximal to the nicotinamide group, and His47 binds the diphosphate of the NAD+ coenzyme. An Asp residue determines the specificity for NAD+ Lactiplantibacillus plantarum WCFS1
1.1.1.90 NADP+ preferred cofactor is NAD+, all of the residues involved in cofactor binding are conserved. An Asp residue determines the specificity for NAD+ Lactiplantibacillus plantarum WCFS1

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.90 Lactiplantibacillus plantarum WCFS1 sequence calculation
-
 5.04