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Literature summary extracted from

  • Xie, X.J.; Huang, W.; Xue, C.Z.; Wei, Q.
    The nonconserved N-terminus of protein phosphatase 2B confers its properties to protein phosphatase 1 (2009), IUBMB Life, 61, 178-183.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.16 chlorogenic acid PP2B but not PP1 is stimulated 4fold by 0.1 mM chlorogenic acid Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 the catalytic subunit of calcineurin is expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.16 additional information the substitution mutant CNA(1-42)-PP1(9-330) has amino acids 1-42 of calcineurin A fused to truncated protein phosphatase 1 with N-terminal residues 1-8 deletion, the mutant is activated by both Mn2+ and Ni2+ and stimulated by chlorogenic acid Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 EDTA isozyme PP1 has low activity after depletion of Mn2+ with EDTA Homo sapiens
3.1.3.16 additional information protein phosphatase 2B is highly resistant to inhibition by okadaic acid Homo sapiens
3.1.3.16 okadaic acid
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Mn2+ isozyme PP1 activity is dependent on Mn2+ with 2.1fold stimulation at 3 mM Homo sapiens
3.1.3.16 Ni2+ PP2B but not PP1 is stimulated by Ni2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 37000
-
 protein phosphatase 1, SDS-PAGE Homo sapiens
3.1.3.16 61000
-
 recombinant catalytic subunit of calcineurin, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphoprotein RII + H2O
-
 Homo sapiens protein RII + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 calcineurin
-
 Homo sapiens
3.1.3.16 CNA calcineurin catalytic subunit Homo sapiens
3.1.3.16 PP1
-
 Homo sapiens
3.1.3.16 PP1c protein phosphatase 1 catalytic subunit Homo sapiens
3.1.3.16 PP2B
-
 Homo sapiens
3.1.3.16 protein phosphatase 1
-
 Homo sapiens
3.1.3.16 protein phosphatase 2B
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.16 0.00003
-
 protein phosphatase 1 Homo sapiens okadaic acid
3.1.3.16 0.00012
-
 mutant enzyme CNA(1-42)-PP1(9-330) Homo sapiens okadaic acid
3.1.3.16 0.005
-
 protein phosphatase 2B Homo sapiens okadaic acid