EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.37 | beta-mercaptoethanol | absolute requirement for activity | Plasmodium falciparum | |
4.1.1.37 | dithiothreitol | absolute requirement for activity | Plasmodium falciparum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.37 | native enzyme and N-terminally truncated UROD are expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.37 | K150T | the mutation does not have a significant effect on Vmax, but decreases Km by 25% | Plasmodium falciparum |
4.1.1.37 | K150T/K219E | the Km of the double mutant decreases by around 40% | Plasmodium falciparum |
4.1.1.37 | K150T/K219E/K277P | the mutant shows no significant change in Km, but the Vmax increases by 3.7fold | Plasmodium falciparum |
4.1.1.37 | K277P | the mutant decreases Km by 40% | Plasmodium falciparum |
EC Number | General Stability | Organism |
---|---|---|
4.1.1.37 | recombinant enzymes are catalytically less efficient compared with the host enzyme | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.37 | Hg2+ | - |
Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.37 | 0.00072 | - |
uroporphyrinogen III | native enzyme | Homo sapiens | |
4.1.1.37 | 0.0087 | - |
uroporphyrinogen III | recombinant enzyme | Plasmodium falciparum | |
4.1.1.37 | 0.0109 | - |
Uroporphyrinogen I | recombinant enzyme | Plasmodium falciparum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.37 | apicoplast | - |
Plasmodium falciparum | 20011 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 41000 | - |
SDS-PAGE | Homo sapiens |
4.1.1.37 | 43000 | - |
SDS-PAGE | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.37 | Homo sapiens | - |
- |
- |
4.1.1.37 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.37 | Ni-NTA column chromatography | Homo sapiens |
4.1.1.37 | Ni-NTA column chromatography | Plasmodium falciparum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.1.37 | erythrocyte | - |
Homo sapiens | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.37 | -20°C or 4°C, 50 mM sodium phosphate buffer, pH 7.5, containing 300 mM imidazole, 1% Triton X-100, 50 mM NaCl, and 10% glycerol, more than a week, significant loss in activity | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.37 | uroporphyrinogen I | - |
Homo sapiens | coproporphyrinogen I + 4 CO2 | - |
? | |
4.1.1.37 | uroporphyrinogen I | - |
Plasmodium falciparum | coproporphyrinogen I + 4 CO2 | - |
? | |
4.1.1.37 | Uroporphyrinogen III | - |
Homo sapiens | Coproporphyrinogen III + 4 CO2 | - |
? | |
4.1.1.37 | Uroporphyrinogen III | - |
Plasmodium falciparum | Coproporphyrinogen III + 4 CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.37 | dimer | Superdex-G200 gel filtration | Homo sapiens |
4.1.1.37 | dimer | Superdex-G200 gel filtration | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.37 | UroD | - |
Homo sapiens |
4.1.1.37 | UroD | - |
Plasmodium falciparum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 37 | - |
recombinant enzyme | Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 7 | - |
pH optimum around 7, recombinant enzyme | Plasmodium falciparum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.1.1.37 | Plasmodium falciparum | calculated from amino acid sequence | - |
8.4 |