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Literature summary extracted from

  • Nagaraj, V.A.; Arumugam, R.; Chandra, N.R.; Prasad, D.; Rangarajan, P.N.; Padmanaban, G.
    Localisation of Plasmodium falciparum uroporphyrinogen III decarboxylase of the heme-biosynthetic pathway in the apicoplast and characterisation of its catalytic properties (2008), Int. J. Parasitol., 39, 559-568.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.37 beta-mercaptoethanol absolute requirement for activity Plasmodium falciparum
4.1.1.37 dithiothreitol absolute requirement for activity Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.37 native enzyme and N-terminally truncated UROD are expressed in Escherichia coli Rosetta2(DE3)pLysS cells Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.37 K150T the mutation does not have a significant effect on Vmax, but decreases Km by 25% Plasmodium falciparum
4.1.1.37 K150T/K219E the Km of the double mutant decreases by around 40% Plasmodium falciparum
4.1.1.37 K150T/K219E/K277P the mutant shows no significant change in Km, but the Vmax increases by 3.7fold Plasmodium falciparum
4.1.1.37 K277P the mutant decreases Km by 40% Plasmodium falciparum

General Stability

EC Number General Stability Organism
4.1.1.37 recombinant enzymes are catalytically less efficient compared with the host enzyme Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.37 Hg2+
-
Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.37 0.00072
-
uroporphyrinogen III native enzyme Homo sapiens
4.1.1.37 0.0087
-
uroporphyrinogen III recombinant enzyme Plasmodium falciparum
4.1.1.37 0.0109
-
Uroporphyrinogen I recombinant enzyme Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.37 apicoplast
-
Plasmodium falciparum 20011
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.37 41000
-
SDS-PAGE Homo sapiens
4.1.1.37 43000
-
SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.37 Homo sapiens
-
-
-
4.1.1.37 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.37 Ni-NTA column chromatography Homo sapiens
4.1.1.37 Ni-NTA column chromatography Plasmodium falciparum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.37 erythrocyte
-
Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
4.1.1.37 -20°C or 4°C, 50 mM sodium phosphate buffer, pH 7.5, containing 300 mM imidazole, 1% Triton X-100, 50 mM NaCl, and 10% glycerol, more than a week, significant loss in activity Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.37 uroporphyrinogen I
-
Homo sapiens coproporphyrinogen I + 4 CO2
-
?
4.1.1.37 uroporphyrinogen I
-
Plasmodium falciparum coproporphyrinogen I + 4 CO2
-
?
4.1.1.37 Uroporphyrinogen III
-
Homo sapiens Coproporphyrinogen III + 4 CO2
-
?
4.1.1.37 Uroporphyrinogen III
-
Plasmodium falciparum Coproporphyrinogen III + 4 CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.37 dimer Superdex-G200 gel filtration Homo sapiens
4.1.1.37 dimer Superdex-G200 gel filtration Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
4.1.1.37 UroD
-
Homo sapiens
4.1.1.37 UroD
-
Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.37 37
-
recombinant enzyme Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.37 7
-
pH optimum around 7, recombinant enzyme Plasmodium falciparum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.37 Plasmodium falciparum calculated from amino acid sequence
-
8.4