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Literature summary extracted from

  • Takahashi, S.; Yonezawa, Y.; Kubota, A.; Ogawa, N.; Maeda, K.; Koshino, H.; Nakata, T.; Yoshida, H.; Mizushina, Y.
    Pyranicin, a non-classical annonaceous acetogenin, is a potent inhibitor of DNA polymerase, topoisomerase and human cancer cell growth (2008), Int. J. Oncol., 32, 451-458.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7
-
 Homo sapiens
2.7.7.7 expressed in Escherichia coli strain JMpbeta5 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 10-epi pyragonicin
-
 Bos taurus
2.7.7.7 10-epi-pyragonicin
-
 Homo sapiens
2.7.7.7 10-epi-pyragonicin
-
 Rattus norvegicus
2.7.7.7 19-epi jimenezin
-
 Bos taurus
2.7.7.7 19-epi-jimenezin
-
 Homo sapiens
2.7.7.7 19-epi-jimenezin
-
 Rattus norvegicus
2.7.7.7 4-hydroxy-5-methyl-3-tetradecyl-dihydrofuran-2(3H)-one
-
 Homo sapiens
2.7.7.7 jimenezin
-
 Bos taurus
2.7.7.7 jimenezin
-
 Homo sapiens
2.7.7.7 jimenezin
-
 Rattus norvegicus
2.7.7.7 additional information not inhibited by gamma-lactone Bos taurus
2.7.7.7 additional information isozyme DNA polymerase lambda is not inhibited by mucocin Homo sapiens
2.7.7.7 additional information not inhibited by gamma-lactone, mucocin, jimenezin, and 19-epi-jimenezin Rattus norvegicus
2.7.7.7 mucocin
-
 Bos taurus
2.7.7.7 mucocin
-
 Rattus norvegicus
2.7.7.7 muconin
-
 Bos taurus
2.7.7.7 muconin
-
 Homo sapiens
2.7.7.7 muconin
-
 Rattus norvegicus
2.7.7.7 pyragonicin
-
 Bos taurus
2.7.7.7 pyragonicin
-
 Homo sapiens
2.7.7.7 pyragonicin
-
 Rattus norvegicus
2.7.7.7 pyranicin potent inhibitor Bos taurus
2.7.7.7 pyranicin
-
 Brassica oleracea
2.7.7.7 pyranicin potent inhibitor Drosophila melanogaster
2.7.7.7 pyranicin
-
 Escherichia coli
2.7.7.7 pyranicin potent inhibitor Homo sapiens
2.7.7.7 pyranicin
-
 Oncorhynchus masou
2.7.7.7 pyranicin potent inhibitor Rattus norvegicus
2.7.7.7 pyranicin
-
 Tequatrovirus T4
2.7.7.7 pyranicin
-
 Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bos taurus
-
-
-
2.7.7.7 Brassica oleracea
-
-
-
2.7.7.7 Drosophila melanogaster
-
-
-
2.7.7.7 Escherichia coli
-
-
-
2.7.7.7 Homo sapiens
-
-
-
2.7.7.7 Oncorhynchus masou
-
 cherry salmon
-
2.7.7.7 Rattus norvegicus
-
-
-
2.7.7.7 Tequatrovirus T4
-
-
-
2.7.7.7 Thermus aquaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Drosophila melanogaster
2.7.7.7
-
 Escherichia coli
2.7.7.7
-
 Homo sapiens
2.7.7.7
-
 Rattus norvegicus
2.7.7.7
-
 Bos taurus
2.7.7.7
-
 Brassica oleracea
2.7.7.7
-
 Oncorhynchus masou

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.7 embryo
-
 Drosophila melanogaster
-
2.7.7.7 testis
-
 Oncorhynchus masou
-
2.7.7.7 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Drosophila melanogaster diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Thermus aquaticus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Rattus norvegicus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Brassica oleracea diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Oncorhynchus masou diphosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase
-
 Drosophila melanogaster
2.7.7.7 DNA polymerase
-
 Homo sapiens
2.7.7.7 DNA polymerase
-
 Brassica oleracea
2.7.7.7 DNA polymerase alpha
-
 Bos taurus
2.7.7.7 DNA polymerase beta
-
 Rattus norvegicus
2.7.7.7 DNA polymerase delta
-
 Oncorhynchus masou
2.7.7.7 DNA polymerase I
-
 Thermus aquaticus
2.7.7.7 DNA polymerase I
-
 Escherichia coli
2.7.7.7 DNA polymerase I
-
 Tequatrovirus T4
2.7.7.7 Klenow fragment
-
 Escherichia coli
2.7.7.7 pol alpha
-
 Bos taurus
2.7.7.7 pol alpha isozyme Drosophila melanogaster
2.7.7.7 pol beta
-
 Rattus norvegicus
2.7.7.7 pol delta
-
 Oncorhynchus masou
2.7.7.7 pol delta isozyme Drosophila melanogaster
2.7.7.7 pol delta isozyme Homo sapiens
2.7.7.7 POl epsilon isozyme Drosophila melanogaster
2.7.7.7 POl epsilon isozyme Homo sapiens
2.7.7.7 Pol eta isozyme Homo sapiens
2.7.7.7 Pol gamma isozyme Homo sapiens
2.7.7.7 Pol I
-
 Thermus aquaticus
2.7.7.7 Pol I
-
 Escherichia coli
2.7.7.7 Pol I
-
 Tequatrovirus T4
2.7.7.7 Pol I isozyme Brassica oleracea
2.7.7.7 Pol II isozyme Brassica oleracea
2.7.7.7 pol iota isozyme Homo sapiens
2.7.7.7 pol kappa isozyme Homo sapiens
2.7.7.7 Pol lambda isozyme Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.7 0.0023
-
 DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.0053
-
 in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Bos taurus pyranicin
2.7.7.7 0.0059
-
 DNA polymerase gamma, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.0065
-
 DNA polymerase alpha, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Drosophila melanogaster pyranicin
2.7.7.7 0.0084
-
 DNA polymerase delta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.0088
-
 in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Oncorhynchus masou pyranicin
2.7.7.7 0.0089
-
 DNA polymerase delta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Drosophila melanogaster pyranicin
2.7.7.7 0.0096
-
 in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Rattus norvegicus pyranicin
2.7.7.7 0.0102
-
 DNA polymerase eta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.0111
-
 DNA polymerase kappa, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.013
-
 DNA polymerase iota, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.014
-
 DNA polymerase epsilon, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Drosophila melanogaster pyranicin
2.7.7.7 0.0158
-
 DNA polymerase epsilon, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Homo sapiens pyranicin
2.7.7.7 0.2
-
 IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Escherichia coli pyranicin
2.7.7.7 0.2
-
 IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Tequatrovirus T4 pyranicin
2.7.7.7 0.2
-
 IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Brassica oleracea pyranicin
2.7.7.7 0.2
-
 IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 74°C Thermus aquaticus pyranicin
2.7.7.7 0.2
-
 IC50 above 0.2 mM, DNA polymerase II, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C Brassica oleracea pyranicin