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Literature summary extracted from

  • Hu, J.; Cheung, N.K.
    Methionine depletion with recombinant methioninase: in vitro and in vivo efficacy against neuroblastoma and its synergism with chemotherapeutic drugs (2009), Int. J. Cancer, 124, 1700-1706.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expressed in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.11 additional information recombinant METase alone inhibits the proliferation of 15/15 NB cell lines in vitro in a dose-dependent manner Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 43000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 QAE-Sepharose column chromatography Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
4.4.1.11 4°C, 20 mM potassium phosphate buffer (pH 7.4) with 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA (pH 8.0), 0.01% (v/v) beta-mercaptoethanol containing 0.3 M NaCl, 2 months, enzyme remains stable Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 L-methionine gamma-lyase
-
 Pseudomonas putida
4.4.1.11 METase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Pseudomonas putida