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Literature summary extracted from

  • Stabeli, R.G.; SantAna, C.D.; Ribeiro, P.H.; Costa, T.R.; Ticli, F.K.; Pires, M.G.; Nomizo, A.; Albuquerque, S.; Malta-Neto, N.R.; Marins, M.; Sampaio, S.V.; Soares, A.M.
    Cytotoxic L-amino acid oxidase from Bothrops moojeni: biochemical and functional characterization (2007), Int. J. Biol. Macromol., 41, 132-140.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.2 additional information the glycosylation site of the enzyme is not important for structural delineation of the active site Bothrops moojeni

Application

EC Number Application Comment Organism
1.4.3.2 medicine LAAO-I exhibited bactericidal, antitumoral, trypanocidal, edematogenic, and platelet-aggregating activities. It induces typical apoptotic DNA fragmentation in HL-60 cells. Potential use of LAAO-I as a therapeutic agent for treatment of diseases in which induction of H2O2 production can be beneficial Bothrops moojeni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 additional information is inactivated by freezing or low pH. Bactericidal, antitumoral, trypanocidal, edematogenic, platelet-aggregating activities, and apoptotic DNA fragmentation in HL-60 cells are inhibited by catalase Bothrops moojeni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 64889
-
 2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni
1.4.3.2 66000
-
 2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni
1.4.3.2 130800
-
 mass spectrometry Bothrops moojeni

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops moojeni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 by ion-exchange chromatography and gel filtration, 12.9fold Bothrops moojeni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops moojeni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 5
-
 with glycine or L-lysine as substrate Bothrops moojeni
1.4.3.2 10
-
 with L-glutamine as substrate Bothrops moojeni
1.4.3.2 35
-
 with L-serine as substrate Bothrops moojeni
1.4.3.2 37.8
-
-
 Bothrops moojeni
1.4.3.2 40
-
 with L-threonine or L-proline as substrate Bothrops moojeni
1.4.3.2 90
-
 with L-arginine as substrate Bothrops moojeni
1.4.3.2 140
-
 with L-histidine as substrate Bothrops moojeni
1.4.3.2 230
-
 with L-alanine as substrate Bothrops moojeni
1.4.3.2 430
-
 with L-valine as substrate Bothrops moojeni
1.4.3.2 435
-
 with L-isoleucine as substrate Bothrops moojeni
1.4.3.2 460
-
 with L-methionine as substrate Bothrops moojeni
1.4.3.2 480
-
 with L-leucine as substrate Bothrops moojeni
1.4.3.2 487.7
-
 12.9fold purified Bothrops moojeni
1.4.3.2 585
-
 with L-tryptophan as substrate Bothrops moojeni
1.4.3.2 635
-
 with L-tyrosine as substrate Bothrops moojeni
1.4.3.2 665
-
 with L-phenylalanine as substrate Bothrops moojeni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 glycine + H2O + O2
-
 Bothrops moojeni ? + NH3 + H2O2
-
 ?
1.4.3.2 L-alanine + H2O + O2
-
 Bothrops moojeni 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2
-
 Bothrops moojeni 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-glutamic acid + H2O + O2
-
 Bothrops moojeni 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-histidine + H2O + O2
-
 Bothrops moojeni 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-isoleucine + H2O + O2 high catalytic activity Bothrops moojeni 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2 high affinity for the substrate Bothrops moojeni 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Bothrops moojeni 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2 high affinity for the substrate Bothrops moojeni 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-norleucine + H2O + O2 high catalytic activity Bothrops moojeni 2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2 high affinity for the substrate Bothrops moojeni phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-proline + H2O + O2
-
 Bothrops moojeni ? + NH3 + H2O2
-
 ?
1.4.3.2 L-serine + H2O + O2
-
 Bothrops moojeni 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-threonine + H2O + O2
-
 Bothrops moojeni 2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-tryptophan + H2O + O2 high affinity for the substrate Bothrops moojeni 3-indole-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-tyrosine + H2O + O2 high affinity for the substrate Bothrops moojeni p-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-valine + H2O + O2
-
 Bothrops moojeni 2-oxoisovalerate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homodimer 2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO-I
-
 Bothrops moojeni

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 4
-
 at temperatures lower than 4°C, enzymatic activity is completely lost Bothrops moojeni

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 4
-
 at a pH below 4, enzymatic activity is completely lost Bothrops moojeni

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Bothrops moojeni isoelectric focusing
-
 4.7