Literature summary extracted from

Barros, N.M.; Nascimento, F.D.; Oliveira, V.; Juliano, M.A.; Juliano, L.; Loisel, T.; Nader, H.B.; Boileau, G.; Tersariol, I.L.; Carmona, A.K.
The critical interaction of the metallopeptidase PHEX with heparan sulfate proteoglycans (2008), Int. J. Biochem. Cell Biol., 40, 2781-2792.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.24.B15	expression in CHO cells	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.24.B15	chondroitin sulfate	-	Homo sapiens
3.4.24.B15	EDTA	5 mM, complete inhibition	Homo sapiens
3.4.24.B15	heparan sulfate	-	Homo sapiens
3.4.24.B15	heparin	-	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.24.B15	endoplasmic reticulum	-	Homo sapiens	5783	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.24.B15	additional information	Homo sapiens	glycosaminoglycans interact with PHEX, interfering with its enzyme activity, protein stability and cellular trafficking. This interaction may regulate PHEX function influencing the mineralization process	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.24.B15	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.24.B15	osteoblast	PHEX colocalizes with heparan sulfate at the cell surface of osteoblasts	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.24.B15	2-aminobenzoyl-Gly-Phe-Ser-Asp-Tyr-Lys(Dnp)-OH + H2O	-	Homo sapiens	2-aminobenzoyl-Gly-Phe-Ser + Asp-Tyr-Lys(Dnp)-OH	-	?
3.4.24.B15	additional information	glycosaminoglycans interact with PHEX, interfering with its enzyme activity, protein stability and cellular trafficking. This interaction may regulate PHEX function influencing the mineralization process	Homo sapiens	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.24.B15	PHEX	-	Homo sapiens

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.4.24.B15	additional information	-	PHEX is protected by glycosaminoglycans against thermal denaturation. The presence of heparin stabilizes the overall PHEX tertiary structure against denaturation at high temperature	Homo sapiens

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.4.24.B15	0.0000025	-	heparin	-	Homo sapiens
3.4.24.B15	0.000306	-	heparan sulfate	-	Homo sapiens
3.4.24.B15	0.000869	-	chondroitin sulfate	-	Homo sapiens

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Release 2023.1 (January 2023)