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Literature summary extracted from

  • Toropainen, M.; Raitolehto, A.; Henckaerts, I.; Wauters, D.; Poolman, J.; Lestrate, P.; Kaeyhty, H.
    Pneumococcal Haemophilus influenzae protein D conjugate vaccine induces antibodies that inhibit glycerophosphodiester phosphodiesterase activity of protein D (2008), Infect. Immun., 76, 4546-4553.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.4.46 medicine to study if membrane protein D (PD)-induced protection against NTHI as a vaccine is due to antibodies that inhibit or neutralize its enzymatic activity, a GlpQ enzyme inhibition assay is developed, and serum samples collected from Finnish infants before and after Pnc-PD vaccination are analyzed for enzyme inhibition and anti-PD IgG antibody concentration. Before vaccination at age 2 months, the majority (84%) of infants has no detectable anti-PD IgG antibodies, and all are enzyme inhibition assay negative. At age 13 to 16 months, all infants receiving three or four doses of Pnc-PD have detectable anti-PD IgG antibodies and 36% of the infants receiving three doses and 26% of the infants receiving four doses of Pnc-PD are inhibition assay positive Haemophilus influenzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Haemophilus influenzae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 L-alpha-glycerophosphorylcholine + H2O
-
 Haemophilus influenzae sn-glycerol 3-phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 GlpQ
-
 Haemophilus influenzae
3.1.4.46 glycerophosphodiester phosphodiesterase
-
 Haemophilus influenzae
3.1.4.46 outer membrane protein D
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.46 22
-
 assay at Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.46 9
-
 assay at Haemophilus influenzae