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Literature summary extracted from
- Composite active site of chondroitin lyase ABC accepting both epimers of uronic acid (2008), Glycobiology, 18, 270-277.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.2.21 | crystal structure identifies additional structurally conserved residues potentially involved in catalysis. A conserved cluster located 12 A from the catalytic tetrad is shown. A His in this cluster is essential for catalysis of dermatane sulfate but not chondroitin sulfate. The enzyme utilizes a single substrate-binding site while having two partially overlapping active sites catalyzing the respective reactions. The spatial separation of the two sets of residues suggests a substrate-induced conformational change that brings all catalytically essential residues close together | Bacteroides thetaiotaomicron |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.2.21 | E628D | mutant shows no activity | Bacteroides thetaiotaomicron |
| 4.2.2.21 | H344A | Km (mM) (chondroitin sulfate A) 0.202, (chondroitin sulfate C) 0.134, kcat (1/sec) (chondroitin sulfate A) 92.66, (chondroitin sulfate B) 35.71, no activity toward dermatan sulfate | Bacteroides thetaiotaomicron |
| 4.2.2.21 | H345A | Km (mM) (chondroitin sulfate A) 0.171, (chondroitin sulfate C) 0.195, kcat (1/sec) (chondroitin sulfate A) 63, (chondroitin sulfate B) 30.88, no activity toward dermatan sulfate | Bacteroides thetaiotaomicron |
| 4.2.2.21 | H453A | Km (mM) (chondroitin sulfate A) 0.038, (chondroitin sulfate C) 0.02, (dermatan sulfate) 0.044, kcat (1/sec) (chondroitin sulfate A) 17, (chondroitin sulfate B) 6.41, (dermatan sulfate) 5.8 | Bacteroides thetaiotaomicron |
| 4.2.2.21 | H454A | mutant shows no activity | Bacteroides thetaiotaomicron |
| 4.2.2.21 | Q173A | Km (mM) (chondroitin sulfate A) 0.102, (chondroitin sulfate C) 0.050, (dermatan sulfate) 0.071, kcat (1/sec) (chondroitin sulfate A) 123, (chondroitin sulfate B) 33, (dermatan sulfate) 6.8 | Bacteroides thetaiotaomicron |
| 4.2.2.21 | R172A | mutant shows no activity | Bacteroides thetaiotaomicron |
| 4.2.2.21 | R267A | Km (mM) (chondroitin sulfate A) 0.109, (chondroitin sulfate C) 0.098, (dermatan sulfate) 0.071, kcat (1/sec) (chondroitin sulfate A) 205, (chondroitin sulfate B) 61.7, (dermatan sulfate) 4.1 | Bacteroides thetaiotaomicron |
| 4.2.2.21 | R514A | mutant shows no activity | Bacteroides thetaiotaomicron |
| 4.2.2.21 | Y461F | mutant shows no activity | Bacteroides thetaiotaomicron |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.21 | 0.02 | - |
chondroitin sulfate C | mutant H453A | Bacteroides thetaiotaomicron |  |
| 4.2.2.21 | 0.033 | - |
chondroitin sulfate C | wild-type | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.038 | - |
chondroitin sulfate A | mutant H453A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.044 | - |
dermatan sulfate | mutant H453A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.05 | - |
chondroitin sulfate C | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.061 | - |
dermatan sulfate | wild-type | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.067 | - |
chondroitin sulfate A | wild-type | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.071 | - |
dermatan sulfate | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.071 | - |
dermatan sulfate | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.098 | - |
chondroitin sulfate C | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.102 | - |
chondroitin sulfate A | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.109 | - |
chondroitin sulfate A | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.134 | - |
chondroitin sulfate C | mutant H344A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.171 | - |
chondroitin sulfate A | mutant H345A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.195 | - |
chondroitin sulfate C | mutant H345A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 0.202 | - |
chondroitin sulfate A | mutant H344A | Bacteroides thetaiotaomicron | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.21 | Ca2+ | addition of Mg2+ or Ca2+ has a dramatically different effect on activity toward CS (glucuronic acid) and DS (iduronic acid). The specific activity for CS substrates increases only moderately (2fold) while an increase of 25fold is observed for DS | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | Mg2+ | addition of Mg2+ or Ca2+ has a dramatically different effect on activity toward CS (glucuronic acid) and DS (iduronic acid). The specific activity for CS substrates increases only moderately (2fold) while an increase of 25fold is observed for DS | Bacteroides thetaiotaomicron | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.21 | Bacteroides thetaiotaomicron | Q8A2I1 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.21 | chondroitin sulfate A | from porcine trachea | Bacteroides thetaiotaomicron | ? | - |
? | |
| 4.2.2.21 | chondroitin sulfate C | from shark cartilage | Bacteroides thetaiotaomicron | ? | - |
? | |
| 4.2.2.21 | dermatan sulfate | from porcine intestinal mucosa | Bacteroides thetaiotaomicron | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.21 | chondroitinase ABC | - |
Bacteroides thetaiotaomicron |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.21 | 37 | - |
assay at | Bacteroides thetaiotaomicron |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.21 | 4.1 | - |
dermatan sulfate | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 5.8 | - |
dermatan sulfate | mutant H453A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 6.41 | - |
chondroitin sulfate C | mutant H453A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 6.8 | - |
dermatan sulfate | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 17 | - |
chondroitin sulfate A | mutant H453A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 30.88 | - |
chondroitin sulfate C | mutant H345A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 33 | - |
chondroitin sulfate C | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 35.71 | - |
chondroitin sulfate C | mutant H344A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 38.45 | - |
dermatan sulfate | wild-type | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 61.7 | - |
chondroitin sulfate C | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 63 | - |
chondroitin sulfate A | mutant H345A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 92.66 | - |
chondroitin sulfate A | mutant H344A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 123 | - |
chondroitin sulfate A | mutant Q173A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 173 | - |
chondroitin sulfate C | wild-type | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 205 | - |
chondroitin sulfate A | mutant R267A | Bacteroides thetaiotaomicron | |
| 4.2.2.21 | 263.2 | - |
chondroitin sulfate A | wild-type | Bacteroides thetaiotaomicron | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.21 | 7.6 | - |
assay at | Bacteroides thetaiotaomicron |
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Release 2023.1 (January 2023)
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