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Literature summary extracted from

  • Tiralongo, J.; Fujita, A.; Sato, C.; Kitajima, K.; Lehmann, F.; Oschlies, M.; Gerardy-Schahn, R.; Muenster-Kuehnel, A.K.
    The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme (2007), Glycobiology, 17, 945-954.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.43 expressed in Escherichia coli and CHO cells Oncorhynchus mykiss
2.7.7.92
-
 Oncorhynchus mykiss

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.92 additional information construction of mutants lacking individual basic amino acids in the N-terminus, disruption of putative nuclear localization signal Oncorhynchus mykiss

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.43 cytoplasm
-
 Oncorhynchus mykiss 5737
-
2.7.7.43 nucleus
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 Oncorhynchus mykiss 5634
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2.7.7.92 nucleus the crucial nuclear localization signal in the fish enzyme is bipartite and the functionality depends on a free N-terminus. Nuclear transport and enzymatic activity are independent functions. The sequence K5K6R7 is sufficient to direct GFP to the nucleus, and is necessary for the nuclear import of the CMP-Sia-synthetase in fish cells Oncorhynchus mykiss 5634
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Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Oncorhynchus mykiss Q90WG6
-
-
2.7.7.92 Oncorhynchus mykiss Q90WG6 cf. EC 2.7.7.43
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Oncorhynchus mykiss diphosphate + CMP-N-acylneuraminate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMP-Sia-syn
-
 Oncorhynchus mykiss
2.7.7.43 CMP-sialic acid synthetase
-
 Oncorhynchus mykiss
2.7.7.92 CMP-Sia synthetase
-
 Oncorhynchus mykiss