BRENDA - Enzyme Database

Comparative survey of putrescine production from agmatine deamination in different bacteria

Landete, J.M.; Arena, M.E.; Pardo, I.; Manca de Nadra, M.C.; Ferrer, S.; Food Microbiol. 25, 882-887 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.3.12
histamine
at concentration of 0.1 g/l about 2% more putrescine production, at concentration of 1 g/l about 5% more putrescine production
Lactobacillus hilgardii
3.5.3.12
histamine
at concentration of 0.1 g/l and 1.0 g/l about 10% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
additional information
diverse polyamines and sugars increased putrescine production, e.g. succinic acid increases putrescine production, but does not alter agmatine deimination, overview
Pseudomonas aeruginosa
3.5.3.12
spermidine
at concentration of 0.1 g/l about 10% more putrescine production, at concentration of 1 g/l 32% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
spermine
at concentration of 0.1 g/l about 2% more putrescine production, at concentration of 1 g/l 23% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
succinate
at concentration of 1 g/l about 5% more putrescine production
Bacillus cereus
3.5.3.12
succinate
at concentration of 0.1 g/l about 15% more putrescine production, at concentration of 1 g/l about 35% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
tyramine
at concentration of 1 g/l about 2% more putrescine production
Lactobacillus hilgardii
3.5.3.12
tyramine
at concentration of 0.1 g/l about 15% more putrescine production, at concentration of 1 g/l 20% more putrescine production
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 80% less putrescine production
Bacillus cereus
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 83% less putrescine production
Enterococcus faecalis
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 75% less putrescine production
Lactobacillus hilgardii
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 68% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
fructose
at concentration of 0.1 g/l about 18% less putrescine production, at concentration of 1 g/l about 60% less putrescine production
Bacillus cereus
3.5.3.12
fructose
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 30% less putrescine production
Enterococcus faecalis
3.5.3.12
fructose
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 75% less putrescine production
Lactobacillus hilgardii
3.5.3.12
fructose
at concentration of 0.1 g/l about 60% less putrescine production, at concentration of 1 g/l 70% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
glucose
at concentration of 0.1 g/l about 22% less putrescine production, at concentration of 1 g/l about 70% less putrescine production
Bacillus cereus
3.5.3.12
glucose
at concentration of 0.1 g/l about 8% less putrescine production, at concentration of 1 g/l about 70% less putrescine production
Enterococcus faecalis
3.5.3.12
glucose
at concentration of 0.1 g/l about 16% less putrescine production, at concentration of 1 g/l about 73% less putrescine production
Lactobacillus hilgardii
3.5.3.12
glucose
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l 19% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
spermidine
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l about 20% less putrescine production
Bacillus cereus
3.5.3.12
spermidine
at concentration of 0.1 g/l about 18% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Enterococcus faecalis
3.5.3.12
spermidine
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 23% less putrescine production
Lactobacillus hilgardii
3.5.3.12
spermine
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l about 20% less putrescine production
Bacillus cereus
3.5.3.12
spermine
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Enterococcus faecalis
3.5.3.12
spermine
at concentration of 0.1 g/l about 20% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Lactobacillus hilgardii
3.5.3.12
succinate
at concentration of 0.1 g/l and 1 g/l only about 2% less putrescine production
Lactobacillus hilgardii
3.5.3.12
tyramine
at concentration of 0.1 g/l about 2% less putrescine production
Lactobacillus hilgardii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Pseudomonas aeruginosa
Pseudomonas aeruginosa is an organism with high putrescine production activity compared to other microorganisms, overview
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Bacillus cereus
-
-
-
3.5.3.12
Bacillus cereus CECT 148
-
-
-
3.5.3.12
Enterococcus faecalis
-
strain ATCC11700
-
3.5.3.12
Enterococcus faecalis ATCC 11700
-
strain ATCC11700
-
3.5.3.12
Lactobacillus hilgardii
-
-
-
3.5.3.12
Lactobacillus hilgardii X1B
-
-
-
3.5.3.12
Pseudomonas aeruginosa
-
-
-
3.5.3.12
Pseudomonas aeruginosa
Q9I6J9
CECT 4122
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.12
additional information
-
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
692430
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
Pseudomonas aeruginosa is an organism with high putrescine production activity compared to other microorganisms, overview
692430
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Pseudomonas aeruginosa
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Enterococcus faecalis
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Bacillus cereus
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Lactobacillus hilgardii
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Bacillus cereus CECT 148
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Enterococcus faecalis ATCC 11700
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Lactobacillus hilgardii X1B
NH3 + N-carbamoylputrescine
-
-
-
ir
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.12
28
-
assay at
Bacillus cereus
3.5.3.12
28
-
assay at
Enterococcus faecalis
3.5.3.12
28
-
assay at
Lactobacillus hilgardii
3.5.3.12
28
-
assay at; in vivo assay at
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
assay at
Bacillus cereus
3.5.3.12
6.5
-
assay at
Enterococcus faecalis
3.5.3.12
6.5
-
assay at
Lactobacillus hilgardii
3.5.3.12
6.5
-
assay at; in vivo assay at
Pseudomonas aeruginosa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.3.12
histamine
at concentration of 0.1 g/l about 2% more putrescine production, at concentration of 1 g/l about 5% more putrescine production
Lactobacillus hilgardii
3.5.3.12
histamine
at concentration of 0.1 g/l and 1.0 g/l about 10% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
additional information
diverse polyamines and sugars increased putrescine production, e.g. succinic acid increases putrescine production, but does not alter agmatine deimination, overview
Pseudomonas aeruginosa
3.5.3.12
spermidine
at concentration of 0.1 g/l about 10% more putrescine production, at concentration of 1 g/l 32% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
spermine
at concentration of 0.1 g/l about 2% more putrescine production, at concentration of 1 g/l 23% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
succinate
at concentration of 1 g/l about 5% more putrescine production
Bacillus cereus
3.5.3.12
succinate
at concentration of 0.1 g/l about 15% more putrescine production, at concentration of 1 g/l about 35% more putrescine production
Pseudomonas aeruginosa
3.5.3.12
tyramine
at concentration of 1 g/l about 2% more putrescine production
Lactobacillus hilgardii
3.5.3.12
tyramine
at concentration of 0.1 g/l about 15% more putrescine production, at concentration of 1 g/l 20% more putrescine production
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 80% less putrescine production
Bacillus cereus
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 83% less putrescine production
Enterococcus faecalis
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 75% less putrescine production
Lactobacillus hilgardii
3.5.3.12
arginine
highest inhibitory effect with 10 g/l arginine, up to 68% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
fructose
at concentration of 0.1 g/l about 18% less putrescine production, at concentration of 1 g/l about 60% less putrescine production
Bacillus cereus
3.5.3.12
fructose
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 30% less putrescine production
Enterococcus faecalis
3.5.3.12
fructose
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 75% less putrescine production
Lactobacillus hilgardii
3.5.3.12
fructose
at concentration of 0.1 g/l about 60% less putrescine production, at concentration of 1 g/l 70% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
glucose
at concentration of 0.1 g/l about 22% less putrescine production, at concentration of 1 g/l about 70% less putrescine production
Bacillus cereus
3.5.3.12
glucose
at concentration of 0.1 g/l about 8% less putrescine production, at concentration of 1 g/l about 70% less putrescine production
Enterococcus faecalis
3.5.3.12
glucose
at concentration of 0.1 g/l about 16% less putrescine production, at concentration of 1 g/l about 73% less putrescine production
Lactobacillus hilgardii
3.5.3.12
glucose
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l 19% less putrescine production
Pseudomonas aeruginosa
3.5.3.12
spermidine
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l about 20% less putrescine production
Bacillus cereus
3.5.3.12
spermidine
at concentration of 0.1 g/l about 18% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Enterococcus faecalis
3.5.3.12
spermidine
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 23% less putrescine production
Lactobacillus hilgardii
3.5.3.12
spermine
at concentration of 0.1 g/l about 10% less putrescine production, at concentration of 1 g/l about 20% less putrescine production
Bacillus cereus
3.5.3.12
spermine
at concentration of 0.1 g/l about 5% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Enterococcus faecalis
3.5.3.12
spermine
at concentration of 0.1 g/l about 20% less putrescine production, at concentration of 1 g/l about 25% less putrescine production
Lactobacillus hilgardii
3.5.3.12
succinate
at concentration of 0.1 g/l and 1 g/l only about 2% less putrescine production
Lactobacillus hilgardii
3.5.3.12
tyramine
at concentration of 0.1 g/l about 2% less putrescine production
Lactobacillus hilgardii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Pseudomonas aeruginosa
Pseudomonas aeruginosa is an organism with high putrescine production activity compared to other microorganisms, overview
N-carbamoylputrescine + NH3
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.12
additional information
-
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
692430
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
Pseudomonas aeruginosa is an organism with high putrescine production activity compared to other microorganisms, overview
692430
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Pseudomonas aeruginosa
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Enterococcus faecalis
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Bacillus cereus
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Lactobacillus hilgardii
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Bacillus cereus CECT 148
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Enterococcus faecalis ATCC 11700
NH3 + N-carbamoylputrescine
-
-
-
ir
3.5.3.12
agmatine + H2O
assay at pH 6.5, 28C, 8 h
692430
Lactobacillus hilgardii X1B
NH3 + N-carbamoylputrescine
-
-
-
ir
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.12
28
-
assay at
Bacillus cereus
3.5.3.12
28
-
assay at
Enterococcus faecalis
3.5.3.12
28
-
assay at
Lactobacillus hilgardii
3.5.3.12
28
-
in vivo assay at
Pseudomonas aeruginosa
3.5.3.12
28
-
assay at
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
assay at
Bacillus cereus
3.5.3.12
6.5
-
assay at
Enterococcus faecalis
3.5.3.12
6.5
-
assay at
Lactobacillus hilgardii
3.5.3.12
6.5
-
in vivo assay at
Pseudomonas aeruginosa
3.5.3.12
6.5
-
assay at
Pseudomonas aeruginosa