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Literature summary extracted from

  • Plaza, L.; Duvetter, T.; Van der Plancken, I.; Meersman, F.; Van Loey, A.; Hendrickx, M.
    Influence of environmental conditions on thermal stability of recombinant Aspergillus aculeatus pectinmethylesterase (2008), Food Chem., 111, 912-920.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 41000
-
SDS-PAGE Aspergillus aculeatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus aculeatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 Superdex-75 gel filtration Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
Aspergillus aculeatus methanol + pectate
-
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Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 Novoshape commercial preparation Aspergillus aculeatus
3.1.1.11 pectinmethylesterase
-
Aspergillus aculeatus
3.1.1.11 PME
-
Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 55 60 at pH 5.0 and a high ionic strength (0.5 M), the enzyme shows a high thermostability (inactivation at temperatures above 60°C), an enhancement of its heat stability is observed at pH 7.0 and temperatures above 55°C, addition of NaCl increases the thermal stability at pH 5.0 and 7.0, while addition of CaCl2 has no influence, regarding the thermal stability in the presence of NaCl at neutral pH, there is complete inactivation at 65°C and no increase of stability at temperatures above 65°C, adding sugars and adding polyols has a positive effect on heat stability Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.11 5 7 at both sides of this pH range the stability decreases slightly Aspergillus aculeatus