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Literature summary extracted from

  • Nishida, M.; Hada, T.; Kuramochi, K.; Yoshida, H.; Yonezawa, Y.; Kuriyama, I.; Sugawara, F.; Yoshida, H.; Mizushina, Y.
    Diallyl sulfides: Selective inhibitors of family X DNA polymerases from garlic (Allium sativum L.) (2008), Food Chem., 108, 551-560.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 Allicin
-
 Homo sapiens
2.7.7.7 Allicin
-
 Rattus norvegicus
2.7.7.7 alliin
-
 Homo sapiens
2.7.7.7 alliin
-
 Rattus norvegicus
2.7.7.7 diallyl pentasulfide
-
 Homo sapiens
2.7.7.7 diallyl pentasulfide
-
 Rattus norvegicus
2.7.7.7 diallyl tetrasulfide
-
 Homo sapiens
2.7.7.7 diallyl tetrasulfide
-
 Rattus norvegicus
2.7.7.7 diallyl trisulfide
-
 Homo sapiens
2.7.7.7 diallyl trisulfide
-
 Rattus norvegicus
2.7.7.7 additional information DNA polymerase alpha is not inhibited by allicin, alliin, diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Bos taurus
2.7.7.7 additional information DNA polymerase isozymes I and II are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Brassica oleracea
2.7.7.7 additional information DNA polymerase isozymes alpha, delta and epsilon are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Drosophila melanogaster
2.7.7.7 additional information DNA polymerase I is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Escherichia coli
2.7.7.7 additional information DNA polymerase lambda is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor Homo sapiens
2.7.7.7 additional information DNA polymerase delta is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Oncorhynchus masou
2.7.7.7 additional information DNA polymerase beta is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor Rattus norvegicus
2.7.7.7 additional information DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Tequatrovirus T4
2.7.7.7 additional information DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Thermus aquaticus
2.7.7.31 diallyl trisulfide
-
 Bos taurus
2.7.7.31 additional information Allium sativa extract is a mixture of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide exhibiting an IC50 value of 0.371 mM at 37°C and a KI value of 0.0121 mM at 37°C Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.7 0.00118
-
 deoxynucleoside triphosphate DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens
2.7.7.7 0.00305
-
 deoxynucleoside triphosphate DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus
2.7.7.31 1.02
-
 dTTP at 37°C Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bos taurus
-
-
-
2.7.7.7 Brassica oleracea
-
-
-
2.7.7.7 Drosophila melanogaster
-
-
-
2.7.7.7 Escherichia coli
-
-
-
2.7.7.7 Homo sapiens
-
-
-
2.7.7.7 Oncorhynchus masou
-
-
-
2.7.7.7 Rattus norvegicus
-
-
-
2.7.7.7 Tequatrovirus T4
-
-
-
2.7.7.7 Thermus aquaticus
-
-
-
2.7.7.31 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Drosophila melanogaster diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Thermus aquaticus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Rattus norvegicus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Brassica oleracea diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Oncorhynchus masou diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 dTTP + DNAn
-
 Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase
-
 Drosophila melanogaster
2.7.7.7 DNA polymerase
-
 Thermus aquaticus
2.7.7.7 DNA polymerase
-
 Escherichia coli
2.7.7.7 DNA polymerase
-
 Tequatrovirus T4
2.7.7.7 DNA polymerase
-
 Brassica oleracea
2.7.7.7 DNA polymerase alpha
-
 Bos taurus
2.7.7.7 DNA polymerase beta
-
 Rattus norvegicus
2.7.7.7 DNA polymerase delta
-
 Oncorhynchus masou
2.7.7.7 DNA polymerase lambda
-
 Homo sapiens
2.7.7.7 Klenow fragment
-
 Escherichia coli
2.7.7.7 Pol
-
 Thermus aquaticus
2.7.7.7 Pol
-
 Tequatrovirus T4
2.7.7.7 pol alpha
-
 Bos taurus
2.7.7.7 pol alpha isozyme Drosophila melanogaster
2.7.7.7 pol beta
-
 Rattus norvegicus
2.7.7.7 pol delta
-
 Oncorhynchus masou
2.7.7.7 pol delta isozyme Drosophila melanogaster
2.7.7.7 POl epsilon isozyme Drosophila melanogaster
2.7.7.7 Pol I isozyme Escherichia coli
2.7.7.7 Pol I isozyme Brassica oleracea
2.7.7.7 Pol II isozyme Brassica oleracea
2.7.7.7 Pol lambda X family DNA polymerase Homo sapiens
2.7.7.31 TdT
-
 Bos taurus
2.7.7.31 terminal deoxynucleotidyl transferase
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.7 additional information
-
 additional information Allium sativum extract has a KI value of 0.00368 mM using DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus
2.7.7.7 additional information
-
 additional information Allium sativum extract has an KI value of 0.0112 mM using DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.7 additional information
-
 Allium sativum extract has an IC50 of 0.0345 mM, using DNA polymerase lambda in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens additional information
2.7.7.7 additional information
-
 Allium sativum extract has an IC50 value of 0.0097 mM using DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus additional information
2.7.7.7 0.115
-
 DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus diallyl trisulfide
2.7.7.7 0.146
-
 DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens diallyl trisulfide
2.7.7.31 0.144
-
 at 37°C Bos taurus diallyl trisulfide