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Literature summary extracted from

  • Barbosa, A.J.; Guimaraes, L.H.; Terenzi, H.F.; Jorge, J.A.; Leone, F.A.; Polizeli, M.L.
    Purification and biochemical characterization of thermostable alkaline phosphatases produced by Rhizopus microsporus var. rhizopodiformis (2008), Folia Microbiol. (Praha), 53, 509-516.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.22
-
 4-nitrophenyl phosphate intracellular form Rhizopus microsporus
3.1.3.1 0.28
-
 4-nitrophenyl phosphate intracellular form Rhizopus microsporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 extracellular
-
 Rhizopus microsporus
-
-
3.1.3.1 intracellular
-
 Rhizopus microsporus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Al3+ activates only the extracellular form Rhizopus microsporus
3.1.3.1 Mg2+ stimulates activity of extra- and intracellular form Rhizopus microsporus
3.1.3.1 Mn2+ stimulates activity of extra- and intracellular form Rhizopus microsporus
3.1.3.1 Na+ stimulates activity of extra- and intracellular form Rhizopus microsporus
3.1.3.1 Zn2+ stimulates activity of extra- and intracellular form Rhizopus microsporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 57000
-
 2 * 57000, SDS-PAGE Rhizopus microsporus
3.1.3.1 118000
-
 gel filtration, intracellular Rhizopus microsporus
3.1.3.1 120000
-
 gel filtration, extracellular Rhizopus microsporus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Rhizopus microsporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 using DEAE-cellulose and ConA-sepharose chromatography Rhizopus microsporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Rhizopus microsporus 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 57000, SDS-PAGE Rhizopus microsporus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 AIP
-
 Rhizopus microsporus
3.1.3.1 alkaline phosphatase
-
 Rhizopus microsporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 65
-
-
 Rhizopus microsporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8
-
-
 Rhizopus microsporus