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Literature summary extracted from

  • Mrdakovic, M.; Lazarevic, J.; Peric-Mataruga, V.; Ilijin, L.; Vlahovic, M.
    Partial characterization of a lipase from gypsy moth (Lymantria dispar L.) larval midgut (2008), Folia Biol. (Krakow), 56, 103-110.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ag+ 7.57% relative activity at 1 mM Lymantria dispar
3.1.1.3 Cu2+ 78.41% relative activity at 1 mM Lymantria dispar
3.1.1.3 Fe2+ 25.54% relative activity at 1 mM Lymantria dispar
3.1.1.3 Fe3+ 58.56% relative activity at 1 mM Lymantria dispar
3.1.1.3 additional information not significantly affected by Na+, K+, Mg2+, Mn2+, Cd2+, Ca2+, and EDTA Lymantria dispar
3.1.1.3 Pb2+ 6.22% relative activity at 1 mM Lymantria dispar
3.1.1.3 phenylmethylsulfonyl fluoride 9.1% relative activity at 1 mM Lymantria dispar

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.31
-
 4-nitrophenyl caprylate in Tris-HCl buffer (50 mM, pH 8.2), at 37°C Lymantria dispar

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information the enzyme is Ca2+ independent Lymantria dispar

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Lymantria dispar
-
 gypsy moth
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 midgut larval midgut Lymantria dispar
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Lymantria dispar 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O best substrate Lymantria dispar 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Lymantria dispar 4-nitrophenol + laurate
-
 ?
3.1.1.3 additional information enzymatic activity chain fatty acid esters longer than C12 is below 10% of maximum activity Lymantria dispar ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 triacylglycerol hydrolase
-
 Lymantria dispar

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 20 77 approximately 10% loss in activity occurrs when the enzyme is kept at 20°C for 1 h, 40% loss of activity at 40°C, the enzyme is almost inactive after preincubation at 70°C, the enzyme is unstable at room temperature Lymantria dispar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.2
-
-
 Lymantria dispar

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 5 8.2 decreased activity is detected after preincubation in buffers of pH below 7.0 and above 8.2, at pH lower than 6.5 the enzyme is almost inactive, complete loss of activity at pH below 5.0 Lymantria dispar