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Literature summary extracted from

  • Engels, V.; Georgi, T.; Wendisch, V.F.
    ScrB (Cg2927) is a sucrose-6-phosphate hydrolase essential for sucrose utilization by Corynebacterium glutamicum (2008), FEMS Microbiol. Lett., 289, 80-89.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B3 fructose 6-phosphate 1.5 fold stimulation, 10 mM Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B3 expressed in Escherichia coli DH5alpha and BL21(DE3), plasmids pGEM-T, pVWEx1, pET16b, recombinant protein and deletion mutant Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B3 Ca2+ CaCl2 inhibits activity of, 5 mM, 10% inhibition Corynebacterium glutamicum
3.2.1.B3 fructose reduced to 70% by the presence of 100 mM Corynebacterium glutamicum
3.2.1.B3 Mn2+ MnCl2 inhibits activity of, 5 mM, 26% inhibition Corynebacterium glutamicum
3.2.1.B3 Zn2+ ZnSO4 inhibits activity of, 5 mM, 98% inhibition Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B3 0.04
-
 sucrose 6-phosphate recombinant protein, pH 6.5, at 30°C Corynebacterium glutamicum
3.2.1.B3 190
-
 sucrose recombinant protein, pH 6.5, at 30°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B3 Mg2+ 20% stimulation by MgSO4, 5 mM Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B3 Corynebacterium glutamicum Q8NMD5 encoded by cg2927 gene that corresponds to the scrB gene; ATCC13032 as wild-type strain, deletion mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B3 gel filtration Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B3 additional information
-
 cg2927 gene corresponds to the scrB gene and encodes sucrose 6-phosphate hydrolase, recombinant protein hydrolyzes sucrose 6-phosphate, sucrose 6-phosphate much more effective than sucrose as substrate, deletion mutant does not grow on sucrose as the sole carbon source, growth in the absence of enzyme activity severely decreased when sucrose is present in addition to glucose, fructose or acetate, transcriptional start sites analyzed, sucrose 6-phosphate hydrolase activity increased during growth in fructose- or sucrose-containing media, overexpression increases hydrolase activity sevenfold, no activity in the scrB deletion mutant Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B3 sucrose + H2O
-
 Corynebacterium glutamicum D-glucose + D-fructose
-
 ?
3.2.1.B3 sucrose 6-phosphate + H2O no activity towards sucrose 6'-phosphate Corynebacterium glutamicum D-glucose 6-phosphate + D-fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B3 scrB protein
-
 Corynebacterium glutamicum
3.2.1.B3 sucrose 6-phosphate hydrolase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B3 45
-
 temperature optimum of ScrB protein Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B3 6.5
-
 assay at, optimum between 6.5 and 7.0 at 30°C Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B3 5 8 13% activity at pH 5.0, 45% activity at pH 8.5 if compared to activity at pH 6.5, no activity below pH 3.0 Corynebacterium glutamicum