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Literature summary extracted from

  • Xie, G.; Liu, M.; Zhu, H.; Lei, B.
    Esterase SeE of Streptococcus equi ssp. equi is a novel nonspecific carboxylic ester hydrolase (2008), FEMS Microbiol. Lett., 289, 181-186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli strain BL21 Streptococcus equi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 diisopropylfluorophosphate complete inhibition at 0.01 mM Streptococcus equi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 3.3
-
 Acetylsalicylic acid in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
3.1.1.1 29
-
 tripropionin in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
3.1.1.1 56
-
 ethyl acetate in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
3.1.1.1 68
-
 vinyl propionate in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi
3.1.1.1 203
-
 Triacetin in 2.0 mM Tris-HCl, pH 7.6, at 25°C Streptococcus equi

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Streptococcus equi B5THG6 subspecies Streptococcus equi equi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography Streptococcus equi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3
-
 using tributyrin as substrate, at 25°C Streptococcus equi
3.1.1.1 24
-
 using vinyl butyrate as substrate, at 25°C Streptococcus equi
3.1.1.1 44
-
 using acetylsalicyclic acid as substrate, at 25°C Streptococcus equi
3.1.1.1 68
-
 using ethyl acetate as substrate, at 25°C Streptococcus equi
3.1.1.1 149
-
 using tripropionin as substrate, at 25°C Streptococcus equi
3.1.1.1 513
-
 using triacetin as substrate, at 25°C Streptococcus equi
3.1.1.1 815
-
 using vinyl propionate as substrate, at 25°C Streptococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Streptococcus equi 4-nitrophenol + acetate
-
 ?
3.1.1.1 acetylsalicylic acid + H2O
-
 Streptococcus equi salicylic acid + acetate
-
 ?
3.1.1.1 ethyl acetate + H2O
-
 Streptococcus equi ethanol + acetate
-
 ?
3.1.1.1 additional information does not hydrolyze ethyl butyrate, trioctanoin, vinyl laurate, acetylcholine, and triglycerides Streptococcus equi ?
-
 ?
3.1.1.1 triacetin + H2O
-
 Streptococcus equi acetic acid + glycerol
-
 ?
3.1.1.1 tributyrin + H2O
-
 Streptococcus equi butanoate + glycerol
-
 ?
3.1.1.1 tripropionin + H2O
-
 Streptococcus equi propionic acid + glycerol
-
 ?
3.1.1.1 vinyl butyrate + H2O
-
 Streptococcus equi vinyl alcohol + butyrate
-
 ?
3.1.1.1 vinyl propionate + H2O
-
 Streptococcus equi vinyl alcohol + propionate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 SeE
-
 Streptococcus equi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 40
-
-
 Streptococcus equi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50 60 the reaction rates dramatically slow down with time at 50 and 60°C Streptococcus equi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
-
 Streptococcus equi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 4 9 the activities of SeE after 1 h incubation at pH 4 and 5 are less than 20% of the activity of SeE after 1 h incubation at pH 7, whereas only a slight decrease is observed after incubation at pH 8 and 9 Streptococcus equi