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Literature summary extracted from

  • Foerster-Fromme, K.; Jendrossek, D.
    Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes for a functional catabolic pathway of methyl-branched compounds (2008), FEMS Microbiol. Lett., 286, 78-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.4 expression of liuA in Escherichia coli strain Rosetta 2 (DE3), subcloning in Escherichia coli strain JM109 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.4 additional information construction of insertion mutants, which show completely impaired growth on all acyclic terpenes tested, i.e.citronellol, geraniol, sodium salts of citronellate and geranylate, and on leucine and isovalerate, phenotype, overview Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.4 0.0023
-
 isovaleryl-CoA pH 7.5, 30°C, recombinant enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.4 41000
-
 4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation Pseudomonas aeruginosa
1.3.8.4 44000
-
 4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation Pseudomonas aeruginosa
1.3.8.4 163000
-
 recombinant enzyme, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + 2,6-dichlorophenolindophenol Pseudomonas aeruginosa high substrate specificity ?
-
 ?
1.3.8.4 additional information Pseudomonas aeruginosa the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.4 Pseudomonas aeruginosa
-
 gene liuA as part of the liuABCDE gene cluster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.4 recombinant LiuA from Escherichia coli Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.4 butyryl-CoA + 2,6-dichlorophenolindophenol high substrate specificity Pseudomonas aeruginosa ?
-
 ?
1.3.8.4 isovaleryl-CoA + 2,6-dichlorophenolindophenol high substrate specificity Pseudomonas aeruginosa ?
-
 ?
1.3.8.4 additional information the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview Pseudomonas aeruginosa ?
-
 ?
1.3.8.4 additional information no activity of LiuA with isobutyryl-CoA, 3-hydroxybutyryl-CoA, octanoyl-CoA, citronellyl-CoA or 5-methyl-hex-4-enoyl-CoA Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.4 tetramer 4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.3.8.4 acyl-CoA dehydrogenase
-
 Pseudomonas aeruginosa
1.3.8.4 LiuA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.4 30
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.4 7.5
-
 assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.4 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa
1.3.8.4 FAD
-
 Pseudomonas aeruginosa