EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.13 | additional information | citrate or phosphoenolpyruvate show no effect on the FBP activity of MJ0400-His6 | Methanocaldococcus jannaschii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.10 | gene Mj0400, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS as mainly insoluble protein | Methanocaldococcus jannaschii |
4.1.2.13 | cloning, overexpression of C-terminally His-tagged MJ0400 enzyme in Escherichia coli Rosetta2(DE3)pLysS cells | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.10 | 4-O-phosphonato-D-erythrose | competitive inhibition of aldolase activity | Methanocaldococcus jannaschii | |
2.2.1.10 | glycylglycine | slightly inhibiting as buffer at 50 mM | Methanocaldococcus jannaschii | |
4.1.2.13 | D-erythrose 4-phosphate | competitive | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.10 | additional information | - |
additional information | MJ0400-His6 exhibits Michaelis-Menten kinetics in the aldolase reaction | Methanocaldococcus jannaschii | |
4.1.2.13 | 0.43 | - |
D-fructose-1,6-bisphosphate | at 50°C | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.10 | L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | Methanocaldococcus jannaschii | - |
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate | - |
? | |
4.1.2.13 | D-fructose-1,6-bisphosphate | Methanocaldococcus jannaschii | - |
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate | - |
r | |
4.1.2.13 | additional information | Methanocaldococcus jannaschii | no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.10 | Methanocaldococcus jannaschii | Q57843 | gene mj0400 | - |
4.1.2.13 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.2.1.10 | soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity | Methanocaldococcus jannaschii |
4.1.2.13 | - |
Methanocaldococcus jannaschii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.2.1.10 | cell culture | optimal growth temperature is 85°C | Methanocaldococcus jannaschii | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.2.1.10 | 0.033 | - |
purified recombinant enzyme, pH 7.5, 50°C, buffer Tris-HCl, HEPES/NaOH, or Na-phosphate | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.10 | L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | - |
Methanocaldococcus jannaschii | 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + 2,3-dioxopropyl phosphate | - |
? | |
2.2.1.10 | additional information | MJ0400 acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, catalyzing the reaction of L-aspartate semialdehyde and 6-deoxy-5-ketofructose-1-phosphate to 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, but the recombinant His6-tagged MJ0400 also catalyzes the cleavage of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, exhibiting fructose-1,6-bisphosphate aldolase, FBP aldolase, activity, EC 4.1.2.13, or the transaldolase reaction with D-fructose-6-phosphate and D-erythose-4-phosphate as substrates. The enzyme shows high substrate specificity for fructose-6-phosphate | Methanocaldococcus jannaschii | ? | - |
? | |
2.2.1.10 | pyruvate + D-erythrose 4-phosphate | - |
Methanocaldococcus jannaschii | ? | - |
? | |
4.1.2.13 | D-fructose-1,6-bisphosphate | - |
Methanocaldococcus jannaschii | glyceraldehyde-3-phosphate + dihydroxyacetone phosphate | - |
r | |
4.1.2.13 | additional information | no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii | Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.10 | 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase | - |
Methanocaldococcus jannaschii |
2.2.1.10 | MJ0400 | - |
Methanocaldococcus jannaschii |
2.2.1.10 | More | cf. EC 4.1.2.13 | Methanocaldococcus jannaschii |
4.1.2.13 | FBP aldolase | - |
Methanocaldococcus jannaschii |
4.1.2.13 | fructose-1,6-bisphosphate aldolase | - |
Methanocaldococcus jannaschii |
4.1.2.13 | MJ0400-His6 | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.10 | 50 | - |
assay at | Methanocaldococcus jannaschii |
4.1.2.13 | 50 | - |
assay at, temperature optimum for the specific acitivity of MJ0400-His6 | Methanocaldococcus jannaschii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.10 | 25 | 85 | 10% activity at 25°C, higher activity at 50°C, optimal activity probably at or above the optimal growth temperature of 85°C | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.10 | 80 | - |
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 37 h | Methanocaldococcus jannaschii |
2.2.1.10 | 100 | - |
10 mg/ml purified recombinant His6-tagged enzyme, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, half-life is 1 h | Methanocaldococcus jannaschii |
4.1.2.13 | additional information | - |
displays heat stability with a half-life of 1 h at 100°C | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.2.1.10 | 7.5 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.10 | 0.38 | - |
4-O-phosphonato-D-erythrose | recombinant enzyme, pH 7.5, 50°C | Methanocaldococcus jannaschii | |
4.1.2.13 | 0.38 | - |
D-erythrose 4-phosphate | - |
Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.2.1.10 | metabolism | the enzyme is involved in both carbon metabolism and amino acid biosynthesis, it catalyzes as transaldolase a step leading to biosynthesis of 3-dehydroquinate, which enters the shikimate pathway, and also shows activity as fructose 1,6-bisphosphate aldolase in carbon metabolism, overview | Methanocaldococcus jannaschii |