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Literature summary extracted from

  • Reher, M.; Gebhard, S.; Schoenheit, P.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophi and Aeropyrum pernix (2007), FEMS Microbiol. Lett., 273, 196-205.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.6 maltose stimulates about 4fold Pyrobaculum aerophilum

Application

EC Number Application Comment Organism
1.2.7.6 degradation Pyrobaculum aerophilum contains a modified Embden-Meyerhof pathway, in which GAPOR replaces the GAPDH/phosphoglycerate kinase couple of the conventional Embden–Meyerhof pathway Pyrobaculum aerophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.6 0.043
-
 D-Glyceraldehyde-3-phosphate
-
 Pyrobaculum aerophilum
1.2.7.6 0.043
-
 D-glyceraldehyde 3-phosphate pH 7.8, 50°C Pyrobaculum aerophilum
1.2.7.6 1
-
 benzyl viologen
-
 Pyrobaculum aerophilum
1.2.7.6 1
-
 Oxidized benzyl viologen pH 7.8, 50°C Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.6 60000
-
 gel filtration Pyrobaculum aerophilum
1.2.7.6 65000
-
 1 * 65000, SDS-PAGE Pyrobaculum aerophilum
1.2.7.6 66000
-
 1 * 66000, SDS-PAGE Pyrobaculum aerophilum
1.2.7.6 70000
-
 PAE1029, represents the GAPOR encoding gene gapor, sequence analysis Pyrobaculum aerophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.6 no activity in Aeropyrum pernix
-
-
-
1.2.7.6 Pyrobaculum aerophilum
-
-
-
1.2.7.6 Pyrobaculum aerophilum Q8ZXY6
-
-
1.2.7.6 Pyrobaculum aerophilum DSM 7523 Q8ZXY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.6
-
 Pyrobaculum aerophilum
1.2.7.6 by gel filtration, 113fold, to homogeneity Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.6 0.15
-
 cell free extract, at 50°C Pyrobaculum aerophilum
1.2.7.6 0.375
-
 at 50°C Pyrobaculum aerophilum
1.2.7.6 17
-
 113fold purified, at 50°C, with a yield of 0.2% Pyrobaculum aerophilum
1.2.7.6 17
-
 pH 7.8, 50°C Pyrobaculum aerophilum

Storage Stability

EC Number Storage Stability Organism
1.2.7.6 -20°C, Tris-HCl buffer, pH 9, several weeks Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.6 D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen
-
 Pyrobaculum aerophilum 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.6 D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen
-
 Pyrobaculum aerophilum DSM 7523 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen is highly specific for glyceraldehyde-3-phosphate Pyrobaculum aerophilum 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.6 additional information does not use glyceraldehyde, acetaldehyde or formaldehyde as substrates Pyrobaculum aerophilum ?
-
 ?
1.2.7.6 additional information enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde Pyrobaculum aerophilum ?
-
 ?
1.2.7.6 additional information enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde Pyrobaculum aerophilum DSM 7523 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.6 monomer 1 * 65000, SDS-PAGE Pyrobaculum aerophilum
1.2.7.6 monomer 1 * 66000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
1.2.7.6 aldehyde ferredoxin oxidoreductase
-
 Pyrobaculum aerophilum
1.2.7.6 GAPOR
-
 Pyrobaculum aerophilum
1.2.7.6 glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Pyrobaculum aerophilum
1.2.7.6 PAE1029
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.6 100
-
 retains 70% of its activity upon incubation for 2 h (half-life, 500 min) Pyrobaculum aerophilum
1.2.7.6 100
-
 2 h, 70% residual activity Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.6 8.1
-
-
 Pyrobaculum aerophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.6 7.7
-
 60% of maximum activity Pyrobaculum aerophilum
1.2.7.6 8.3
-
 60% of maximum activity Pyrobaculum aerophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.7.6 7.7 8.3 60% activity at pH 7.7 and 8.3 Pyrobaculum aerophilum

General Information

EC Number General Information Comment Organism
1.2.7.6 metabolism extracts of maltose/yeast extract/nitrate-grown cells contain all enzyme activities of a modified Embden-Meyerhof pathway, including ATP-dependent glucokinase, phosphoglucose isomerase, ATP-dependent 6-phosphofructokinase, fructose-1,6-phosphate aldolase, triose-phosphate isomerase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, phosphoglycerate mutase, enolase and pyruvate kinase Pyrobaculum aerophilum