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Literature summary extracted from

  • Greig, N.; Wyllie, S.; Patterson, S.; Fairlamb, A.H.
    A comparative study of methylglyoxal metabolism in trypanosomatids (2009), FEBS J., 276, 376-386.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 Trypanosoma brucei lacks GLO1 activity. GLO1 of Trypanosoma cruzi is overexpressed in Trypanosoma brucei in the procyclic and the bloodstream form to complete the glyoxalase system, resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstrating that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 16000
-
 Western blot analysis of whole cell lysate, equivalent to the predicted molecular mass Leishmania major
4.4.1.5 16000
-
 Western blot analysis of whole cell lysate, equivalent to the predicted molecular mass. Recombinant protein containing a tetracycline-inducible pLew100-TcGLO1 construct, expression of GLO1 of Trypanosoma cruzi in Trypanosoma brucei, Western blot analysis Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Trypanosoma brucei
-
 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Trypanosoma cruzi
-
 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Leishmania major
-
 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Leishmania major Friedlin
-
 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 additional information Trypanosoma brucei the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview ?
-
 ?
1.2.1.22 additional information Trypanosoma cruzi the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview ?
-
 ?
1.2.1.22 additional information Leishmania major the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview ?
-
 ?
1.2.1.22 additional information Leishmania major Friedlin the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.283 Leishmania major
-
-
-
1.1.1.283 Leishmania major Friedlin
-
-
-
1.1.1.283 Trypanosoma brucei
-
-
-
1.1.1.283 Trypanosoma cruzi
-
-
-
1.2.1.22 Leishmania major
-
-
-
1.2.1.22 Trypanosoma brucei
-
-
-
1.2.1.22 Trypanosoma cruzi
-
-
-
4.4.1.5 Leishmania major
-
-
-
4.4.1.5 no activity in Trypanosoma brucei
-
 overexpression of glyoxalase I from Trypanosomas cruzi exhibits enzyme activity in Trypanosomas brucei. The wild-type Trypansoma brucei lacks the GLO1 activity. No apparent GLO1 gene can be identified in the genome
-
4.4.1.5 Trypanosoma cruzi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.5 (R)-S-lactoylglutathione = glutathione + 2-oxopropanal comparative study of methylgloxal metabolism in trypanosomatids: Trypanosoma brucei, Trypanosoma cruzi und Leishmania major. Trypanosoma brucei lacks GLO1 activity. Overexpression of GLO1 from Trypanosoma cruzi in Trypanosoma brucei results in restoration of the glyoxalase system resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstratong that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Trypanosoma cruzi
a
4.4.1.5 (R)-S-lactoylglutathione = glutathione + 2-oxopropanal comparative study of methylgloxal metabolism in trypanosomatids: Trypanosoma brucei, Trypanosoma cruzi und Leishmania major. Trypanosoma brucei lacks GLO1 activity. Overexpression of GLO1 from Trypanosoma cruzi in Trypanosoma brucei results in restoration of the glyoxalase system resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstratong that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo Leishmania major
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.22 additional information no activity in the bloodstream stage of the parasite Trypanosoma brucei
-
1.2.1.22 procyclic form
-
 Trypanosoma brucei
-
1.2.1.22 trypanosomoid form
-
 Trypanosoma brucei
-
1.2.1.22 trypanosomoid form
-
 Trypanosoma cruzi
-
1.2.1.22 trypanosomoid form
-
 Leishmania major
-
4.4.1.5 epimastigote
-
 Trypanosoma cruzi
-
4.4.1.5 additional information in the bloodstream and procyclic form of the recombinant protein of Trpypanosomas brucei, overexpressed with GLO1 of Trypanosomas cruzi Trypanosoma cruzi
-
4.4.1.5 promastigote
-
 Leishmania major
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.283 0.0048
-
 at 25°C, in 50 mM HEPES (pH 7.0) Trypanosoma cruzi
1.1.1.283 0.0053
-
 at 25°C, in 50 mM HEPES (pH 7.0) Leishmania major
1.1.1.283 0.0094
-
 at 25°C, in 50 mM HEPES (pH 7.0) Trypanosoma brucei
1.2.1.22 0.00048
-
 whole cell lysate Trypanosoma cruzi
1.2.1.22 0.00051
-
 whole cell lysate Leishmania major
1.2.1.22 0.00051
-
 whole cell lysate, procyclics Trypanosoma brucei
4.4.1.5 0.023
-
 recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, procyclic form, pH 7.0, 25°C Trypanosoma cruzi
4.4.1.5 0.038
-
 recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, bloodstream form, pH 7.0, 25°C Trypanosoma cruzi
4.4.1.5 0.042
-
 whole cell lysate, pH 7.0, 25°C Trypanosoma cruzi
4.4.1.5 0.085
-
 whole cell lysate, pH 7.0, 25°C Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.283 methylglyoxal + NADPH + H+
-
 Trypanosoma brucei L-lactaldehyde + NADP+
-
 ?
1.1.1.283 methylglyoxal + NADPH + H+
-
 Trypanosoma cruzi L-lactaldehyde + NADP+
-
 ?
1.1.1.283 methylglyoxal + NADPH + H+
-
 Leishmania major L-lactaldehyde + NADP+
-
 ?
1.1.1.283 methylglyoxal + NADPH + H+
-
 Leishmania major Friedlin L-lactaldehyde + NADP+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Trypanosoma brucei (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Trypanosoma cruzi (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Leishmania major (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Leishmania major Friedlin (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 additional information the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview Trypanosoma brucei ?
-
 ?
1.2.1.22 additional information the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview Trypanosoma cruzi ?
-
 ?
1.2.1.22 additional information the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview Leishmania major ?
-
 ?
1.2.1.22 additional information the enzyme shows NAD+-dependent l-lactaldehyde dehydrogenase activity in the methylglyoxyl metabolism, metabolizing methylglyoxal via methylglyoxal reductase and lactaldehyde dehydrogenase to L-lactate, overview Leishmania major Friedlin ?
-
 ?
4.4.1.5 trypanothione + methylglyoxal cell lysate of the recombinant protein, esxpression of GLO1 from Trypanosomas cruzi in Trypanosomas brucei which lacks GLO1 activity, pH 7.0, 25°C Trypanosoma cruzi S,S'-bis((R)-lactoyl)trypanothione the product formation is measured by monitoring at 240 nm ?
4.4.1.5 trypanothione + methylglyoxal cell lysate, pH 7.0, 25°C Leishmania major S,S'-bis((R)-lactoyl)trypanothione the formation is measured by monitoring at 240 nm ?
4.4.1.5 trypanothione + methylglyoxal cell lysate, pH 7.0, 25°C Leishmania major Friedlin S,S'-bis((R)-lactoyl)trypanothione the formation is measured by monitoring at 240 nm ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.283 MeGR
-
 Trypanosoma brucei
1.1.1.283 MeGR
-
 Trypanosoma cruzi
1.1.1.283 MeGR
-
 Leishmania major
1.1.1.283 NADPH-dependent methylglyoxal reductase
-
 Trypanosoma brucei
1.1.1.283 NADPH-dependent methylglyoxal reductase
-
 Trypanosoma cruzi
1.1.1.283 NADPH-dependent methylglyoxal reductase
-
 Leishmania major
1.2.1.22 L-lactaldehyde dehydrogenase
-
 Trypanosoma brucei
1.2.1.22 L-lactaldehyde dehydrogenase
-
 Trypanosoma cruzi
1.2.1.22 L-lactaldehyde dehydrogenase
-
 Leishmania major
1.2.1.22 LADH
-
 Trypanosoma brucei
1.2.1.22 LADH
-
 Trypanosoma cruzi
1.2.1.22 LADH
-
 Leishmania major
4.4.1.5 Glo1
-
 Trypanosoma cruzi
4.4.1.5 Glo1
-
 Leishmania major
4.4.1.5 glyoxalase I
-
 Trypanosoma cruzi
4.4.1.5 glyoxalase I
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.5 25
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7
-
 assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 NAD+ dependent on Trypanosoma brucei
1.2.1.22 NAD+ dependent on Trypanosoma cruzi
1.2.1.22 NAD+ dependent on Leishmania major