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Literature summary extracted from

  • Meier, T.I.; Yan, D.; Peery, R.B.; McAllister, K.A.; Zook, C.; Peng, S.B.; Zhao, G.
    Identification and characterization of an inhibitor specific to bacterial NAD+-dependent DNA ligases (2008), FEBS J., 275, 5258-5271.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BL21 (DE3) cells Homo sapiens
6.5.1.2 expressed in Escherichia coli BL21 (DE3) cells Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 additional information not inhibited by 2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine, trimethoprim, and methotrexate Homo sapiens
6.5.1.1 additional information not inhibited by 2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine, trimethoprim, and methotrexate Tequatrovirus T4
6.5.1.2 2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine IC50 of 0.0001 mg/ml, specific inhibitor of NAD+-dependent DNA ligase, inhibits competitively with respect to NAD+ and specifically inhibits enzyme adenylation, but not DNA adenylation or ligation Streptococcus pneumoniae
6.5.1.2 methotrexate IC50 of 0.008 mg/ml Streptococcus pneumoniae
6.5.1.2 additional information not inhibited by trimethoprim Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.0021
-
 NAD+
-
 Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 72000
-
 SDS-PAGE Streptococcus pneumoniae
6.5.1.2 72130
-
 calculated from amino acid sequence Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Homo sapiens
-
-
-
6.5.1.1 Tequatrovirus T4
-
-
-
6.5.1.2 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 Sepharose column chromatography and hydroxyapatite column chromatography Homo sapiens
6.5.1.1 Sepharose column chromatography and hydroxyapatite column chromatography Tequatrovirus T4
6.5.1.2 Sepharose column chromatography and hydroxyapatite column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Streptococcus pneumoniae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.2 ? x * 72000, SDS-PAGE Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 Homo sapiens
6.5.1.1 Lig I
-
 Homo sapiens
6.5.1.1 T4 DNA ligase
-
 Tequatrovirus T4
6.5.1.2 LigA
-
 Streptococcus pneumoniae
6.5.1.2 NAD+-dependent DNA ligase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.2 0.1
-
 NAD+ pH 8.0, 21°C Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Homo sapiens
6.5.1.1 ATP
-
 Tequatrovirus T4
6.5.1.2 NAD+
-
 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.5.1.2 0.0014
-
 2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine
-
 Streptococcus pneumoniae