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Literature summary extracted from

  • Bardales, J.R.; Hellman, U.; Villamarin, J.A.
    Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis (2008), FEBS J., 275, 4479-4489.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 PKI(5-24)
-
 Mytilus galloprovincialis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 0.0125
-
 Kemptide C-subunit isoform C5, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis
2.7.11.11 0.0125
-
 Kemptide C-subunit isoform C6, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis
2.7.11.11 0.0147
-
 Kemptide C-subunit isoform C3, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis
2.7.11.11 0.0158
-
 Kemptide C-subunit isoform C1, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis
2.7.11.11 0.0186
-
 Kemptide C-subunit isoform C4, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis
2.7.11.11 0.0247
-
 Kemptide C-subunit isoform C2, in 50 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol, 5 mM MgCl2, 0.2 mM ATP, at 25°C Mytilus galloprovincialis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.11 41300
-
 C-subunit isoform C4, estimated by SDS-PAGE Mytilus galloprovincialis
2.7.11.11 41900
-
 C-subunit isoform C1, estimated by SDS-PAGE Mytilus galloprovincialis
2.7.11.11 42800
-
 C-subunit isoform C3, estimated by SDS-PAGE Mytilus galloprovincialis
2.7.11.11 42800
-
 C-subunit isoform C5, estimated by SDS-PAGE Mytilus galloprovincialis
2.7.11.11 43400
-
 C-subunit isoform C2, estimated by SDS-PAGE Mytilus galloprovincialis
2.7.11.11 44500
-
 C-subunit isoform C6, estimated by SDS-PAGE Mytilus galloprovincialis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Mytilus galloprovincialis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 DEAE-cellulose chromatography and Mono-S column chromatography Mytilus galloprovincialis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 mantle contains PKA C-subunit isoforms C4, C5, and C6 Mytilus galloprovincialis
-
2.7.11.11 muscle posterior adductor muscle, contains PKA C-subunit isoforms C1, C2, and C3 Mytilus galloprovincialis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide
-
 Mytilus galloprovincialis ADP + Kemptide phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 tetramer
-
 Mytilus galloprovincialis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 cAMP-dependent protein kinase catalytic subunit (C-subunit)
-
 Mytilus galloprovincialis
2.7.11.11 PKA
-
 Mytilus galloprovincialis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Mytilus galloprovincialis
2.7.11.11 cAMP dependent Mytilus galloprovincialis