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Literature summary extracted from
- S-nitrosylated proteins of a medicinal CAM plant Kalanchoe pinnata ribulose-1,5-bisphosphate carboxylase/oxygenase activity targeted for inhibition (2008), FEBS J., 275, 2862-2872.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.39 | S-nitrosoglutathione | inactivation by nitrosylation | Kalanchoe pinnata |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.1.39 | S-nitrosoproteome | - |
Kalanchoe pinnata | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.39 | Mg2+ | - |
Kalanchoe pinnata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.39 | 14400 | - |
Rubisco small subunit, rbcS, determined by SDS-PAGE and silver staining, verified by MALDI-TOF mass spectrometry | Kalanchoe pinnata |
| 4.1.1.39 | 20190 | - |
Rubisco small subunit, precursor, identified by MALDI-TOF mass spectrometry | Kalanchoe pinnata |
| 4.1.1.39 | 20350 | - |
Rubisco small subunit chain 2B, chloroplast precursor, identified by MALDI-TOF mass spectrometry | Kalanchoe pinnata |
| 4.1.1.39 | 48850 | - |
Rubisco large subunit, identified by MALDI-TOF mass spectrometry | Kalanchoe pinnata |
| 4.1.1.39 | 55000 | - |
Rubisco large subunit, rbcL, determined by SDS-PAGE and silver staining, verified by MALDI-TOF mass spectrometry | Kalanchoe pinnata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.39 | 3-phospho-D-glycerate + H+ | Kalanchoe pinnata | - |
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.39 | Kalanchoe pinnata | I1UMG7 | Rubisco large subunit, small subunit, precursor, small subunit chain 2B, chloroplast precursor, identified by MALDI-TOF mass spectrometry | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.1.1.39 | nitrosylation | - |
Kalanchoe pinnata |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.39 | to identify the S-nitrosylated proteins, neutravidin affinity chromatography is used to purify biotinylated proteins from Kalanchoe pinnata leaf extracts | Kalanchoe pinnata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.1.39 | leaf | - |
Kalanchoe pinnata | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.39 | 3-phospho-D-glycerate + H+ | - |
Kalanchoe pinnata | D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.39 | ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Kalanchoe pinnata |
| 4.1.1.39 | Rubisco | - |
Kalanchoe pinnata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.39 | 30 | - |
activity assay | Kalanchoe pinnata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.39 | 8 | - |
activity assay | Kalanchoe pinnata |
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