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Literature summary extracted from

  • Murray-Stewart, T.; Wang, Y.; Goodwin, A.; Hacker A.; Meeker, A.; casero, R.A.
    Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology (2008), FEBS J., 275, 2795-2806.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.16 SMO/PAOh1 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme Homo sapiens
1.5.3.16 SMO5 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.0005
-
 spermine splice variant SMO5 Homo sapiens
1.5.3.16 0.0006
-
 spermine SMO/PAOh1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.16 cytosol SMO/PAOh1 Homo sapiens 5829
-
1.5.3.16 cytosol SMO5 Homo sapiens 5829
-
1.5.3.16 nucleus SMO/PAOh1 Homo sapiens 5634
-
1.5.3.16 nucleus SMO5 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.16 61900
-
 x * 61900, SMO/PAOh1, SDS-PAGE Homo sapiens
1.5.3.16 65000
-
 x * 65000, SMO5, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Homo sapiens
-
-
-
1.5.3.16 Homo sapiens Q9NWM0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16 recombinant enzyme, SMO/PAOh1 Homo sapiens
1.5.3.16 recombinant enzyme, SMO5 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 additional information SMO/PAOh1 exhibits no oxidase activity when using N1,N12-diacetylspermine, N1-acetylspermidine, N8-acetylspermidine, spermidine, or the polyamine analogues, bis(ethyl)norspermine or N1-ethyl-N11-(cyclopropyl)methyl-4,8,diazaundecane Homo sapiens ?
-
 ?
1.5.3.16 additional information splice variant SMO5 exhibits no oxidase activity when using N1,N12-diacetylspermine, N1-acetylspermidine, N8-acetylspermidine, spermidine, or the polyamine analogues, bis(ethyl)norspermine or N1-ethyl-N11-(cyclopropyl)methyl-4,8,diazaundecane Homo sapiens ?
-
 ?
1.5.3.16 N1-acetylspermine + O2 + H2O less than 10% of the activity with spermine, SMO/PAOh1 Homo sapiens ?
-
 ?
1.5.3.16 N1-acetylspermine + O2 + H2O less than 10% of the activity with spermine, splice variant SMO5 Homo sapiens ?
-
 ?
1.5.3.16 spermine + O2 + H2O exhibits a strong preference for spermine as the primary substrate over all other naturally occurring polyamines, SMO/PAOh1 Homo sapiens ?
-
 ?
1.5.3.16 spermine + O2 + H2O exhibits a strong preference for spermine as the primary substrate over all other naturally occurring polyamines, splice variant SMO5 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.16 ? x * 61900, SMO/PAOh1, SDS-PAGE Homo sapiens
1.5.3.16 ? x * 65000, SMO5, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 SMO/PAOh1
-
 Homo sapiens
1.5.3.16 SMO5 splice variant protein Homo sapiens
1.5.3.16 spermine oxidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 0.28
-
 N1-acetylspermine SMO/PAOh1 Homo sapiens
1.5.3.16 0.28
-
 N1-acetylspermine splice variant SMO5 Homo sapiens
1.5.3.16 3.11
-
 spermine splice variant SMO5 Homo sapiens
1.5.3.16 7.55
-
 spermine SMO/PAOh1 Homo sapiens