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Literature summary extracted from

  • Rauch, G.; Ehammer, H.; Bornemann, S.; Macheroux, P.
    Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity (2008), FEBS J., 275, 1464-1473.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.5 mutant proteins are heterologously expressed in Escherichia coli, strain BL21(DE3)RP Neurospora crassa

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.5 S127A amino acid replacements are performed using the QuikChange site-directed mutagenesis kit Neurospora crassa
4.2.3.5 S16A amino acid replacements are performed using the QuikChange site-directed mutagenesis kit Neurospora crassa
4.2.3.5 S16A/S127A double-mutant protein Neurospora crassa

General Stability

EC Number General Stability Organism
4.2.3.5 apparent stability of all mutant proteins is comparable to that of the wild-type enzyme Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.5 0.0027
-
5-enolpyruvylshikimate 3-phosphate wild-type enzyme Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.5 5-enolpyruvylshikimate 3-phosphate Neurospora crassa last enzyme of the shikimate pathway, catalyzes the anti-1,4-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate chorismate + phosphate chorismate is a precursor for the biosynthesis of aromatic compounds r
4.2.3.5 additional information Neurospora crassa the dissociation constant for the S127A mutant protein is similar to that of the wild-type enzyme, whereas the replacement of S16 with alanine results in a slight increase of the dissociation constant ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.5 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.5 by two-step chromatographic procedure Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.5 cell culture
-
Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.5 additional information
-
activity of mutant S127A is 6fold lower than that of the wild-type protein Neurospora crassa
4.2.3.5 additional information
-
activity of mutant S16A is 70fold lower than that of the wild-type protein Neurospora crassa
4.2.3.5 additional information
-
chorismate synthase has an intrinsic NADPH: FMN oxidoreductase activity that enables the enzyme to generate the reduced FMN cofactor (bifunctionality) Neurospora crassa
4.2.3.5 additional information
-
no activity of double-mutant protein S16A/S127A, replacement of both serine residues produces a synergistic effect Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.5 5-enolpyruvylshikimate 3-phosphate last enzyme of the shikimate pathway, catalyzes the anti-1,4-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate Neurospora crassa chorismate + phosphate chorismate is a precursor for the biosynthesis of aromatic compounds r
4.2.3.5 additional information the dissociation constant for the S127A mutant protein is similar to that of the wild-type enzyme, whereas the replacement of S16 with alanine results in a slight increase of the dissociation constant Neurospora crassa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.5 chorismate synthase
-
Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.5 25
-
assay at Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.5 additional information
-
additional information value of double-mutant S16A/S127A with 5-enolpyruvylshikimate 3-phosphate as substrate is below the detection limit Neurospora crassa
4.2.3.5 0.012
-
5-enolpyruvylshikimate 3-phosphate mutant S16A Neurospora crassa
4.2.3.5 0.14
-
5-enolpyruvylshikimate 3-phosphate mutant S127A Neurospora crassa
4.2.3.5 0.87
-
5-enolpyruvylshikimate 3-phosphate wild-type enzyme Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.5 7.5
-
assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.5 FMN reduced, the S16A/S127A double-mutant protein showy slightly weaker binding of the cofactor Neurospora crassa