BRENDA - Enzyme Database

Glutamate recognition and hydride transfer by Escherichia coli glutamyl-tRNA reductase

Lueer, C.; Schauer, S.; Virus, S.; Schubert, W.D.; Heinz, D.W.; Moser, J.; Jahn, D.; FEBS J. 274, 4609-4614 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
wild-type GluTR and variants produced as N-terminal His6-fusion proteins
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
C50S
inactive
Escherichia coli
1.2.1.70
E54K
retains 6% reductase and 2% esterase activity
Escherichia coli
1.2.1.70
H99N
retains 5% reductase and 4% esterase activity
Escherichia coli
1.2.1.70
Q116L
lacks reductase activity whereas 30% esterase activity is retained
Escherichia coli
1.2.1.70
R52K
retains 5% reductase and 4% esterase activity
Escherichia coli
1.2.1.70
R52Q
inactive
Escherichia coli
1.2.1.70
S109A
retains 28% reductase and 30% esterase activity
Escherichia coli
1.2.1.70
T49V
retains 10% reductase and 5% esterase activity
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Escherichia coli
P0A6X1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
wild-type GluTR and variants
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutaminyl-tRNAGlu + NADPH + H+
-
692283
Escherichia coli
? + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
692283
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
wild-type GluTR and variants produced as N-terminal His6-fusion proteins
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
C50S
inactive
Escherichia coli
1.2.1.70
E54K
retains 6% reductase and 2% esterase activity
Escherichia coli
1.2.1.70
H99N
retains 5% reductase and 4% esterase activity
Escherichia coli
1.2.1.70
Q116L
lacks reductase activity whereas 30% esterase activity is retained
Escherichia coli
1.2.1.70
R52K
retains 5% reductase and 4% esterase activity
Escherichia coli
1.2.1.70
R52Q
inactive
Escherichia coli
1.2.1.70
S109A
retains 28% reductase and 30% esterase activity
Escherichia coli
1.2.1.70
T49V
retains 10% reductase and 5% esterase activity
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
wild-type GluTR and variants
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutaminyl-tRNAGlu + NADPH + H+
-
692283
Escherichia coli
? + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
692283
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?