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Literature summary extracted from

  • Urushibata, Y.; Ebisu, S.; Matsui, I.
    A thermostable dolichol phosphoryl mannose synthase responsible for glycoconjugate synthesis of the hyperthermophilic archaeon Pyrococcus horikoshii (2008), Extremophiles, 12, 665-676.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.83 phosphatidylcholine relative activity: 122% Pyrococcus horikoshii
2.4.1.83 phosphatidylethanolamine relative activity: 125% Pyrococcus horikoshii
2.4.1.83 phosphatidylglycerol relative activity: 356% Pyrococcus horikoshii
2.4.1.83 phosphatidylinositol relative activity: 70% Pyrococcus horikoshii
2.4.1.83 phosphatidylserine relative activity: 342% Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.83 full-length protein is expressed in Escherichia coli and the N-terminal domain is expressed in Saccharomyces cerevisiae Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.83 D38A 5.8% of the activity of wild-type Pyrococcus horikoshii
2.4.1.83 D39A 2% of the activity of wild-type Pyrococcus horikoshii
2.4.1.83 D89A 0.09% of the activity of wild-type Pyrococcus horikoshii
2.4.1.83 D91A 0.17% of the activity of wild-type Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.83 phosphatidylinositol relative activity: 70% Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.00059
-
 C110-dolichyl phosphate
-
 Pyrococcus horikoshii
2.4.1.83 0.00117
-
 C55-undecaprenyl phosphate
-
 Pyrococcus horikoshii
2.4.1.83 0.00159
-
 C120-dolichyl phosphate
-
 Pyrococcus horikoshii
2.4.1.83 0.0023
-
 C85-dolichyl phosphate
-
 Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.83 membrane N-terminal domain is membrane-bound Pyrococcus horikoshii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Mg2+ required Pyrococcus horikoshii
2.4.1.83 Mn2+ required Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.83 41010
-
 deduced from cDNA Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83 solubilized with Triton X-100 and purified to homogeneity Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDP-mannose + C110-dolichyl phosphate
-
 Pyrococcus horikoshii GDP + C110-dolichyl D-mannosyl phosphate
-
 ?
2.4.1.83 GDP-mannose + C120-dolichyl phosphate
-
 Pyrococcus horikoshii GDP + C120-dolichyl D-mannosyl phosphate
-
 ?
2.4.1.83 GDP-mannose + C55-undecaprenyl phosphate
-
 Pyrococcus horikoshii GDP + C55-undecaprenyl D-mannosyl phosphate
-
 ?
2.4.1.83 GDP-mannose + C85-dolichyl phosphate
-
 Pyrococcus horikoshii GDP + C85-dolichyl D-mannosyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.83 dolichol phosphoryl mannose synthase
-
 Pyrococcus horikoshii
2.4.1.83 DPM synthase
-
 Pyrococcus horikoshii
2.4.1.83 PH0051
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.83 60
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.83 75
-
 protein shows thermostability up to 75°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 8.5
-
 assay at Pyrococcus horikoshii