Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stefanidi, E.; Vorgias, C.E.
    Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme (2008), Extremophiles, 12, 541-552.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 additional information the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF Moritella marina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chi60, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Moritella marina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 allosamidin 50% inhibition at 0.0005 mM Moritella marina
3.2.1.14 additional information the enzyme is not affected by various bivalent cations and EDTA, DTT, iodoacetic acid, and PMSF Moritella marina
3.2.1.14 Urea 30% inhibition at 5 M Moritella marina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.101
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 0°C, purified recombinant enzyme Moritella marina
3.2.1.14 0.37
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 10°C, purified recombinant enzyme Moritella marina
3.2.1.14 0.416
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 15°C, purified recombinant enzyme Moritella marina
3.2.1.14 0.5
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 5°C or 20°C, purified recombinant enzyme Moritella marina
3.2.1.14 0.555
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 28°C, purified recombinant enzyme Moritella marina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 additional information the enzyme is not affected by various bivalent cations Moritella marina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 60000
-
gel filtration Moritella marina
3.2.1.14 60788
-
1 * 60788, Chi60, sequence calculation Moritella marina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Moritella marina chitinases hydrolyse the beta-1,4-linkages in chitin ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Moritella marina B1VBB0 i.e. Vibrio marinus, gene chi60
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant Chi60 25fold from Escherichia coli Moritella marina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.15
-
purified recombinant enzyme, substrate powdered chitosan Moritella marina
3.2.1.14 0.16
-
purified recombinant enzyme, substrate colloidal chitin Moritella marina
3.2.1.14 0.91
-
purified recombinant enzyme, substrate 4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose Moritella marina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-1,4-N,N'-diacetyl-chitobiose + H2O
-
Moritella marina 4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose
-
?
3.2.1.14 4-nitrophenyl beta-1,4-N,N'-diacetylchitobiose + H2O
-
Moritella marina ?
-
?
3.2.1.14 chitin + H2O chitinases hydrolyse the beta-1,4-linkages in chitin Moritella marina ?
-
?
3.2.1.14 colloidal chitin + H2O
-
Moritella marina ?
-
?
3.2.1.14 additional information the enzyme is not active with synthetic 4-nitrophenyl-linked glycosidic substrates, overview Moritella marina ?
-
?
3.2.1.14 powdered chitosan + H2O
-
Moritella marina ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 60788, Chi60, sequence calculation Moritella marina
3.2.1.14 More Chi60 domain structure, overview Moritella marina

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 MmChi60
-
Moritella marina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 28
-
assay at Moritella marina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14
-
60
-
Moritella marina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 additional information
-
the enzyme stability at 18°C and 28°C is similar to the stability at 4°C Moritella marina
3.2.1.14
-
30 purified recombinant enzyme, 72 h, completely stable Moritella marina
3.2.1.14 40
-
purified recombinant enzyme, half-life is 72 h Moritella marina
3.2.1.14 50
-
purified recombinant enzyme, half-life is 5 h Moritella marina
3.2.1.14 60
-
purified recombinant enzyme, half-life is 10 min Moritella marina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.79
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 0°C, purified recombinant enzyme Moritella marina
3.2.1.14 4.33
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 5°C, purified recombinant enzyme Moritella marina
3.2.1.14 5.46
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 10°C, purified recombinant enzyme Moritella marina
3.2.1.14 7.33
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 15°C, purified recombinant enzyme Moritella marina
3.2.1.14 11.88
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 20°C, purified recombinant enzyme Moritella marina
3.2.1.14 19.54
-
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose pH 5.0, 28°C, purified recombinant enzyme Moritella marina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
assay at Moritella marina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 10
-
Moritella marina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3
-
purified recombinant enzyme, 3 days, loss of 80% of activity at 4°C Moritella marina
3.2.1.14 4 10 the purified recombinant Chi60 exhibits broad pH stability maintaining over 60% activity after 10 days at a pH range at 4°C Moritella marina
3.2.1.14 8 10 purified recombinant enzyme, 10 days, no loss of activity at 4°C Moritella marina