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Literature summary extracted from

  • Serra, M.P.; Senn, A.M.; Algranati, I.D.
    Post-translational processing, metabolic stability and catalytic efficiency of oat arginine decarboxylase expressed in Trypanosoma cruzi epimastigotes (2008), Exp. Parasitol., 122, 169-176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.19 MG-132 ADC proteins are degraded by ubiquitin-dependent mechanisms carried out by 26S proteasome, the major degradation pathway for soluble proteins. Experiments carry out with ADC-transgenic cultures of Trypanosoma cruzi incubated with the peptide aldehyde proteasome inhibitor MG-132 show a marked increase of ADC activity probably due to the reduction of the enzyme turnover rate as shown after blocking protein synthesis with cycloheximide. Avena sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.19 oat enzyme is expressed in Trypanosoma cruzi epimastigotes after transfection with a C-terminal six-His tag and as untagged enzyme, active ADC is expressed in the parasites and the primary translational product is cleaved into 2 polypeptides through a proteolytic process. When the his-tag is added to the C-terminal end of ADC it causes very significant changes on the metabolic stability and catalytic parameters of the heterologous enzyme expressed in the transformed parasites. Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.19 alpha-difluoromethylarginine
-
 Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.19 0.11
-
 L-arginine untagged ADC Avena sativa
4.1.1.19 0.31
-
 L-arginine His-tagged ADC Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.19 24000
-
 1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis Avena sativa
4.1.1.19 42000
-
 1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis Avena sativa
4.1.1.19 66000
-
 inactive precursor protein, active recombinant ADC is formed in vivo by a complex of two His-tagged polypeptide chains of 42 and 24 kDa by a subsequent incubation during different times with extracts of Arabidopsis seedlings which contain a proteolytic activity, processing of oat ADC does not require a specific protease Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.19 L-arginine Avena sativa first reaction of a putrescine synthetic pathway found in bacteria and plants agmatine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Avena sativa P22220
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.19 proteolytic modification post-translational proteolysis of ADC depends on the presence of a protease-sensitive loop in the structure of the protein Avena sativa

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19 ADC protein attached to the six-His tag at the C-terminal end is purified from parasite extracts by immobilized metal affinity chromatography through a Ni2+-agarose column Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.19 additional information
-
 enzyme activities of extracts obtained from ADC or ADCH-transfected parasites are higher in the presence of oxidized glutathione than under reducing conditions with dithiothreitol in the reaction mixture Avena sativa
4.1.1.19 0.0012
-
 untagged ADC Avena sativa
4.1.1.19 0.0331
-
 His-tagged ADC Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine first reaction of a putrescine synthetic pathway found in bacteria and plants Avena sativa agmatine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.19 heterodimer 1 * 42000 + 1 * 24000, determined by SDS-polyacrylamide gel electrophoresis Avena sativa

Synonyms

EC Number Synonyms Comment Organism
4.1.1.19 ADC
-
 Avena sativa
4.1.1.19 arginine decarboxylase
-
 Avena sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.19 37
-
 assay at Avena sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.19 additional information
-
 additional information To ascertain whether the ADC enzyme expressed in transgenic Trypanosoma cruzi has a modified metabolic turnover when attached to the His-tag, cycloheximide is added to parasite cultures transformed with plasmids to stop protein synthesis. ADC half-life inside the parasite is 150 min, the enzyme attached to the His-tail is more stable, with a half-life of about 14 h. Avena sativa
4.1.1.19 0.9
-
 L-arginine untagged ADC Avena sativa
4.1.1.19 9.9
-
 L-arginine His-tagged ADC Avena sativa

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.19 pyridoxal 5'-phosphate ADC belongs to the group IV pyridoxal 5'-phosphate-dependent decarboxylases Avena sativa