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Literature summary extracted from

  • Folbergrova, J.; Jesina, P.; Drahota, Z.; Lisy, V.; Haugvicova, R.; Vojtiskova, A.; Houstek, J.
    Mitochondrial complex I inhibition in cerebral cortex of immature rats following homocysteic acid-induced seizures (2007), Exp. Neurol., 204, 597-609.
    View publication on PubMed

Application

EC Number Application Comment Organism
7.1.1.2 medicine inhibition of complex I may elicit enhanced formation of reactive oxygen species and contribute thus to neuronal injury Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 DL-homocysteic acid marked (ca. 64%) decrease of respiratory chain complex I activity in the cerebral cortex of immature rats following seizures induced by bilateral intracerebroventricular infusion of DL-homocysteic acid (600 nanomol/side). Decrease is already evident during the acute phase of seizures (60-90 min after infusion) and persists for at least 20 h after the seizures. Inhibition is selective for complex I since activities of complex II and IV and citrate synthase remain unaffected. Inhibition of complex I activity is not associated with changes in complex I content. Enhanced production of reactive oxygen species by inhibited complex I in mitochondria from DL-homocysteic acid-treated animals. Competitive NMDA receptor antagonist AP7, a selective and potent group II mGluR agonist (2R,4R)-APDC and a highly selective group III mGluR, subtype 8, agonist (S)-3,4-DCPG, significantly reduce the extent of complex I inhibition. The superoxide dismutase mimetic Tempol and a selective peroxynitrite scavenger and decomposition catalyst FeTPPS provide a significant attenuation of complex I inhibition associated with DL-homocysteic acid-induced seizures Rattus norvegicus
7.1.1.2 rotenone rotenone-induced increment of H2O2 production is 2fold higher upon DL-homocysteic acid-treatment, thus clearly indicating elevated production of reactive oxygen species at complex I Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Rattus norvegicus
-
immature 12-day-old male Wistar albino rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 cerebral cortex
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 35.91
-
ca. 20 h survival after seizures Rattus norvegicus
7.1.1.2 36.28
-
acute phase of seizures Rattus norvegicus
7.1.1.2 100.6
-
controls Rattus norvegicus
7.1.1.2 102
-
controls Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 glutamate
-
Rattus norvegicus ?
-
?
7.1.1.2 malate
-
Rattus norvegicus ?
-
?
7.1.1.2 NADH + decyl-ubiquinone + H+
-
Rattus norvegicus NAD+ + decyl-ubiquinol
-
?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
Rattus norvegicus
7.1.1.2 NADH-ubiquinone oxidoreductase
-
Rattus norvegicus