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Literature summary extracted from

  • Cote, A.; Shareck, F.
    Cloning, purification and characterization of two lipases from Streptomyces coelicolor A3(2) (2008), Enzyme Microb. Technol., 42, 381-388.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ag+ isoyzme SCO1725 shows 11% residual activity in the presence of 1 mM Ag+; isozyme SCO7513 shows 10% residual activity in the presence of 1 mM Ag+ Streptomyces coelicolor
3.1.1.3 Ca2+ isoyzme SCO1725 shows 91% residual activity at 10 mM Ca2+; isozyme SCO7513 shows 86% residual activity in the presence of 1 mM Ca2+ Streptomyces coelicolor
3.1.1.3 Co2+ isoyzme SCO1725 shows 93% residual activity in the presence of 10 mM Co2+; isozyme SCO7513 shows 72% residual activity in the presence of 10 mM Co2+ Streptomyces coelicolor
3.1.1.3 Cu2+ isoyzme SCO1725 shows 88% residual activity at 1 mM Cu2+; isozyme SCO7513 shows 51% residual activity in the presence of 10 mM Cu2+ Streptomyces coelicolor
3.1.1.3 dithiothreitol isoyzme SCO1725 shows 36% residual activity in the presence of 10 mM dithiothreitol; isozyme SCO7513 shows 86% residual activity in the presence of 10 mM dithiothreitol Streptomyces coelicolor
3.1.1.3 Mg2+ isoyzme SCO1725 shows 92% residual activity in the presence of 10 mM Mg2+; isozyme SCO7513 shows 83% residual activity in the presence of 10 mM Mg2+ Streptomyces coelicolor
3.1.1.3 additional information Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride; Ca2+ and Mg2+ do not significantly reduce the lipolytic activity, resistant to inhibition by phenylmethylsulfonyl fluoride Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 23980
-
 SCO1725, estimated molecular weight Streptomyces coelicolor
3.1.1.3 26970
-
 SCO7513, estimated molecular weight Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Streptomyces coelicolor Q93J06
-
-
3.1.1.3 Streptomyces coelicolor Q9S2A5
-
-
3.1.1.3 Streptomyces coelicolor A3(2) Q93J06
-
-
3.1.1.3 Streptomyces coelicolor A3(2) Q9S2A5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 Q Sepharose column chromatography, CM Sepharose FF column chromatography, and DEAE Sepharose column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Streptomyces coelicolor A3(2) 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Streptomyces coelicolor A3(2) 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Streptomyces coelicolor A3(2) 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + stearate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Streptomyces coelicolor A3(2) 4-nitrophenol + stearate
-
 ?
3.1.1.3 additional information isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 Streptomyces coelicolor ?
-
 ?
3.1.1.3 additional information isozyme SCO1725 shows no activity on tricaprin, tricaproin, and olive oil, 4-nitrophenyl stearate is hardly hydrolysed, 4-nitrophenyl stearate is no substrate for isozyme SCO1725 Streptomyces coelicolor A3(2) ?
-
 ?
3.1.1.3 tributyrin + H2O partially hydrolysed by isozyme SCO1725 Streptomyces coelicolor dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O partially hydrolysed by isozyme SCO1725 Streptomyces coelicolor A3(2) dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O
-
 Streptomyces coelicolor dicaprin + decaprate
-
 ?
3.1.1.3 tricaprylin + H2O
-
 Streptomyces coelicolor dicaprylin + caprylate
-
 ?
3.1.1.3 tricaprylin + H2O
-
 Streptomyces coelicolor A3(2) dicaprylin + caprylate
-
 ?
3.1.1.3 Tween 80 + H2O
-
 Streptomyces coelicolor ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Streptomyces coelicolor
3.1.1.3 SCO1725 isozyme Streptomyces coelicolor
3.1.1.3 SCO7513 isozyme Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 20 30 isozyme SCO1725 Streptomyces coelicolor
3.1.1.3 45 55 isozyme SCO7513 Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 70 isozyme SCO1725 is stable when incubated for 30 min at temperatures in the range of 30-70°C (at pH 7) Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7.5 9 isozyme SCO1725 Streptomyces coelicolor
3.1.1.3 8.5 10 isozyme SCO7513 Streptomyces coelicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 10 11 the isozyme SCO1725 becomes unstable at pH over 10, where the activity drops to 34% at pH 10.5 and to 8% at pH 11 Streptomyces coelicolor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Streptomyces coelicolor isozyme SCO7513, predicted value
-
 4.73
3.1.1.3 Streptomyces coelicolor isozyme SCO1725, predicted value
-
 6.23