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Literature summary extracted from

  • Lee, D.C.; Zheng, J.; She, Y.M.; Jia, Z.
    Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism (2008), EMBO J., 27, 1758-1766.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.10.2 additional information protein kinase activation through an intra-molecular autophosphorylation process Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.10.2 the Etk C-terminal kinase domain, 451-726, and Y574A, R614A mutants are cloned into pET vectors for expression in Escherichia coli BL21DE3 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.10.2 the C-terminal kinase domain of Escherichia coli Tyr kinase Etk is solved at 2.5 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.10.2 R614A mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 R614K mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 Y574A mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 Y574E mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 Y574F mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 Y574G mutant, used in autokinase activity assay Escherichia coli
2.7.10.2 Y574N mutant, used in autokinase activity assay Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.10.2 inner membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.10.2 ATP + [protein]-L-tyrosine Escherichia coli
-
 ADP + [protein]-L-tyrosine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.2 Escherichia coli P58764
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.10.2 phosphoprotein
-
 Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.10.2 by nickel nitrilotriacetate agarose affinity chromatography and gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.10.2 additional information
-
 wild type activity 100%, mutant Y574A 98.4%, mutant Y574E 100%, mutant Y574F 12.1%, mutant Y574G 86.7%, mutant Y574N 26.9%, mutant R614A 14.6%, mutant R614K 61.8% Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.2 ATP + [protein]-L-tyrosine
-
 Escherichia coli ADP + [protein]-L-tyrosine phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.10.2 tyrosine kinase Etk
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.10.2 22
-
 kinase activity assay at room temperature Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.10.2 8.5
-
 kinase activity assay Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.10.2 ATP
-
 Escherichia coli