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Literature summary extracted from

  • Mailloux, R.J.; Darwich, R.; Lemire, J.; Appanna, V.
    The monitoring of nucleotide diphosphate kinase activity by blue native polyacrylamide gel electrophoresis (2008), Electrophoresis, 29, 1484-1489.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 membrane
-
Pseudomonas fluorescens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+
-
Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 additional information
-
the enzyme has a molecular weight of 60-120 kDa Pseudomonas fluorescens
2.7.4.6 15000
-
x * 15000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Pseudomonas fluorescens
-
strain ATCC 13525
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + CDP
-
Pseudomonas fluorescens ADP + CTP
-
r
2.7.4.6 ATP + dCDP
-
Pseudomonas fluorescens ADP + dCTP
-
r
2.7.4.6 ATP + dGDP
-
Pseudomonas fluorescens ADP + dGTP
-
r
2.7.4.6 ATP + dTDP
-
Pseudomonas fluorescens ADP + dTTP
-
r
2.7.4.6 ATP + GDP
-
Pseudomonas fluorescens ADP + GTP
-
r
2.7.4.6 ATP + UDP
-
Pseudomonas fluorescens ADP + UTP
-
r
2.7.4.6 additional information coupling of the formation of ATP to phenazine methosulfate and iodonitrotetrazolium chloride, coupling is achieved using hexokinase and G6PDH, which produce the necessary NADPH molecules for formazan precipitation at the site of enzyme activity, method optimization, overview, measurement of the reverse enzyme reactions, overview Pseudomonas fluorescens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 More the enzyme has a molecular weight of 60-120 kDa Pseudomonas fluorescens
2.7.4.6 oligomer x * 15000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK
-
Pseudomonas fluorescens
2.7.4.6 nucleotide diphosphate kinase
-
Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 7.4
-
assay at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
Pseudomonas fluorescens