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Literature summary extracted from
- Expression of alkaline sphingomyelinase in yeast cells and anti-inflammatory effects of the expressed enzyme in a rat colitis model (2008), Digest. Dis. Sci., 54, 1440-1448.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | taurocholate | the enzyme activity is specifically stimulated by 6 mM taurocholate | Homo sapiens |  |
| 3.1.4.12 | taurodeoxycholate | the enzyme activity is specifically stimulated by about 2.5 mM taurodeoxycholate | Homo sapiens | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | medicine | intrarectal instillation of recombinant human Alk-SMase once daily for 1 week significantly reduces the inflammation score and protects the colonic epithelium from inflammatory destruction in a rat colitis model | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | expressed in Pichia pastoris strain GS115 | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Triton X-100 | strong inhibition in the presence of 0.05-0.2% Triton X-100 | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 60000 | - |
SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | SE column chromatography, the enzyme is not retained in DEAE-Sepharose column equilibrated with 20 mM Tris-HCl buffer containing 0.075 M NaCl | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | intestine | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 0.027 | - |
recombinant human Alk-SMase, in 50 mM Tris | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | alk-SMase | key enzyme in the intestinal tract for digestion of dietary sphingomyelin | Homo sapiens |
| 3.1.4.12 | alkaline sphingomyelinase | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 9 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7 | 10 | the activity of Alk-SMase expressed from the yeast has a similar pH dependency, being low at acid pH, increasing after 7 and optimal at 9.0 | Homo sapiens |
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Release 2023.1 (January 2023)
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