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Literature summary extracted from

  • Asthana, R.K.; Nigam, S.; Maurya, A.; Kayastha, A.M.; Singh, S.P.
    Trehalose-producing enzymes MTSase and MTHase in Anabaena 7120 under NaCl stress (2008), Curr. Microbiol., 56, 429-435.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.15 NaCl exposure to 150 mM NaCl for 48 h causes increased expression of MTSase Anabaena sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.15 Na+ triggers maximum expression and activity of MTSase Anabaena sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 114000
-
 SDS-PAGE Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O Anabaena sp. enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
 r
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O Anabaena sp. 7120 enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Anabaena sp.
-
 7120, facing NaCl stress, grown in BG-11 medium free from combined nitrogen sources, continuous tungsten plus fluorescent illumination for trehalose hyperproduction
-
5.4.99.15 Anabaena sp.
-
-
-
5.4.99.15 Anabaena sp. 7120
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.141 additional information
-
 trehalose formation (combined action of MTHase and maltooligosyltrehalose synthase) upon NaCl exposure for 36 h: 0.069 mg/h/ml, 48 h: 0.117 mg/h/ml, 54 h: 0.094 mg/h/ml Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase Anabaena sp. (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
 r
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase Anabaena sp. 7120 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
 r
5.4.99.15 maltohexaose
-
 Anabaena sp. alpha-maltotetraosyl trehalose
-
 ?
5.4.99.15 maltohexaose
-
 Anabaena sp. 7120 alpha-maltotetraosyl trehalose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.141 maltooligosyltrehalose trehalohydrolase
-
 Anabaena sp.
3.2.1.141 MTHase
-
 Anabaena sp.
5.4.99.15 maltooligosyltrehalose synthase
-
 Anabaena sp.
5.4.99.15 MTSase
-
 Anabaena sp.