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Literature summary extracted from

  • Ueda, M.; Asano, T.; Nakazawa, M.; Miyatake, K.; Inouye, K.
    Purification and characterization of novel raw-starch-digesting and cold-adapted alpha-amylases from Eisenia foetida (2008), Comp. Biochem. Physiol. B, 150, 125-130.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.1 industry could be useful in industrial operations such as brewing and food processing, due to their activity at low and high temperatures Eisenia fetida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Al3+ 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Ba2+ 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Co2+ 1 mM, pH 8.0, 24 h at 4°C, 92% and 93% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Cu2+ 1 mM, pH 8.0, 24 h at 4°C, 36% and 64% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 EDTA 1 mM, pH 8.0, 24 h at 4°C, 98% and 76% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Fe2+ 1 mM, pH 8.0, 24 h at 4°C, 68% and 24% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Hg2+ 1 mM, pH 8.0, 24 h at 4°C, 35% and 31% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Pb2+ 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Zn2+ 1 mM, pH 8.0, 24 h at 4°C, 94% and 64% residual activity for Amy I and Amy II, respectively Eisenia fetida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ag+ 1 mM, pH 8.0, 24 h at 4°C, slight activation for Amy I and Amy II Eisenia fetida
3.2.1.1 Al3+ 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Ba2+ 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Ca2+ 1 mM, pH 8.0, 24 h at 4°C, 105% and 106% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Mg2+ 1 mM, pH 8.0, 24 h at 4°C, 111% and 107% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Mn2+ 1 mM, pH 8.0, 24 h at 4°C, 108% and 102% residual activity for Amy I and Amy II, respectively Eisenia fetida
3.2.1.1 Pb2+ 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively Eisenia fetida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 60000
-
 SDS-PAGE Eisenia fetida

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Eisenia fetida
-
 earthworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 Amy I, 85.2fold purified and Amy II, 263fold purified Eisenia fetida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
 18.7 U/mg, purified Amy II Eisenia fetida
3.2.1.1 additional information
-
 6.05 U/mg, purifed Amy II Eisenia fetida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 additional information no activity detected using cassava starch as substrate for Amy I Eisenia fetida ?
-
 ?
3.2.1.1 starch + H2O various sources of starch are tested, japonica rice as control with 100% relative activity, India rice: 294% and 508% relative activity for Amy I and Amy II, respectively, corn: 10.7% and 2.8% relative activity for Amy I and Amy II, respectively, wheat: 27.1% and 47.5% relative activity for Amy I and Amy II, respectively, potato: 10.6% and 4.47% relative activity for Amy I and Amy II, respectively, sweet potato: 13.5% and 1.88% relative activity for Amy I and Amy II, respectively, cassava: 1.22% relative activity for Amy II Eisenia fetida D-glucose + maltose + maltotriose reactions product identified by thin-layer-chromatography ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 60000, SDS-PAGE Eisenia fetida

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 Amy I
-
 Eisenia fetida
3.2.1.1 Amy II
-
 Eisenia fetida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 37
-
 assay at Eisenia fetida
3.2.1.1 50
-
 Amy I and Amy II Eisenia fetida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 20 70 Amy I and Amy II, 20°C: ca. 40% and ca. 50% relative activity of Amy I and Amy II, respectively, 70°C: ca. 60% and ca. 40% relative activity of Amy I and Amy II, respectively Eisenia fetida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 10 60 Amy I and Amy II, pH 8.0, 30 min, 10°C: ca. 90% relative activity for both Amy I and Amy II, 60°C: ca. 10% and ca. 90% relative activity for Amy I and Amy II, respectively, maximal stability between 50 and 60°C Eisenia fetida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5
-
 assay at, optimum for Amy I and Amy II, showing at least 95% of their maximum activities over a pH range from pH 7.0 to 9.0 Eisenia fetida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4 9 Amy I, pH 4: ca. 30% relative activity, pH 9: ca. 40% relative activity Eisenia fetida
3.2.1.1 5 7 Amy II, pH 5.0: ca. 50% relative activity, pH 7.0: ca. 40% relative activity Eisenia fetida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5 9 Amy II, 24 h at 4°C, pH 5, ca. 42% residual activity, pH 9: ca. 75% residual activity, maximal stability between pH 7 and 9 Eisenia fetida
3.2.1.1 5 10 Amy I, 24 h at 4°C, pH 5, ca. 22% residual activity, pH 10: ca. 10% residual activity, maximal stability between pH 7 and 9 Eisenia fetida