Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, H.; Li, Y.; Chen, W.; Cai, B.
    A novel salicylaldehyde dehydrogenase-NahV involved in catabolism of naphthalene from Pseudomonas putida ND6 (2007), Chin. Sci. Bull., 52, 1942-1948.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.2.1.65 additional information two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria Pseudomonas putida
1.2.1.65 additional information two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.65 nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) Pseudomonas putida
1.2.1.65 nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.65 Al3+ inhibits NahF activity by 42%; inhibits NahV activity by 15% Pseudomonas putida
1.2.1.65 Ba2+ inhibits NahF activity by 62%; inhibits NahV activity by 52% Pseudomonas putida
1.2.1.65 Ca2+ inhibits NahF activity by 63%; inhibits NahV activity by 35% Pseudomonas putida
1.2.1.65 Cs+ inhibits NahF activity by 44%; inhibits NahV activity by 69% Pseudomonas putida
1.2.1.65 Cu2+ inhibits NahF activity by 70% Pseudomonas putida
1.2.1.65 Li+ inhibits NahF activity by 50%; inhibits NahV activity by 57% Pseudomonas putida
1.2.1.65 Mg2+ inhibits NahF activity by 34%; inhibits NahV activity by 36% Pseudomonas putida
1.2.1.65 Ni2+ inhibits NahF activity by 60%; inhibits NahV activity by 70% Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.65 0.00016
-
NAD+
-
Pseudomonas putida
1.2.1.65 0.00109
-
NAD+
-
Pseudomonas putida
1.2.1.65 0.034
-
Salicylaldehyde
-
Pseudomonas putida
1.2.1.65 0.146
-
Salicylaldehyde
-
Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.65 Cu2+ enhances NahV activity by 63% activity Pseudomonas putida
1.2.1.65 Fe2+ enhances NahF activity by 40% activity Pseudomonas putida
1.2.1.65 Fe2+ enhances NahV activity by 152% Pseudomonas putida
1.2.1.65 additional information Zn2+ has no effect on NahF activity Pseudomonas putida
1.2.1.65 Zn2+ enhances NahV activity by 14% activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.65 50000
-
1 * 50000, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.65 Pseudomonas putida Q6XUJ4
-
-
1.2.1.65 Pseudomonas putida Q6XUN9
-
-
1.2.1.65 Pseudomonas putida ND6 Q6XUJ4
-
-
1.2.1.65 Pseudomonas putida ND6 Q6XUN9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.65 His-tagged NahF purified by Ni-NTA chromatography Pseudomonas putida
1.2.1.65 His-tagged NahV purified by Ni-NTA chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.65 5-chlorosalicylaldehyde + NAD+ + H2O
-
Pseudomonas putida 5-chlorosalicylate + NADH + H+
-
?
1.2.1.65 5-chlorosalicylaldehyde + NAD+ + H2O
-
Pseudomonas putida ND6 5-chlorosalicylate + NADH + H+
-
?
1.2.1.65 caprylic aldehyde + NAD+ + H2O
-
Pseudomonas putida caprylic acid + NADH + H+
-
?
1.2.1.65 caprylic aldehyde + NAD+ + H2O
-
Pseudomonas putida ND6 caprylic acid + NADH + H+
-
?
1.2.1.65 formaldehyde + NAD+ + H2O
-
Pseudomonas putida formate + NADH + H+
-
?
1.2.1.65 formaldehyde + NAD+ + H2O
-
Pseudomonas putida ND6 formate + NADH + H+
-
?
1.2.1.65 glutaraldehyde + NAD+ + H2O
-
Pseudomonas putida glutarate + NADH + H+
-
?
1.2.1.65 glutaraldehyde + NAD+ + H2O
-
Pseudomonas putida ND6 glutarate + NADH + H+
-
?
1.2.1.65 glyoxal + NAD+ + H2O
-
Pseudomonas putida glyoxylate + NADH + H+
-
?
1.2.1.65 m-nitrobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida m-nitrobenzoate + NADH + H+
-
?
1.2.1.65 o-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida o-methoxybenzoate + NADH + H+
-
?
1.2.1.65 o-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida ND6 o-methoxybenzoate + NADH + H+
-
?
1.2.1.65 o-nitrobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida o-nitrobenzoate + NADH + H+
-
?
1.2.1.65 salicylaldehyde + NAD+ + H2O best substrate Pseudomonas putida salicylate + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.65 monomer 1 * 50000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.1.65 NahF
-
Pseudomonas putida
1.2.1.65 NahV
-
Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.65 50
-
72.8% activity is retained for NahF after a 30 min exposure Pseudomonas putida
1.2.1.65 50
-
NahV looses 88.8% activity after a 30 min exposure Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.65 NAD+
-
Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.65 Pseudomonas putida
-
-
5.08
1.2.1.65 Pseudomonas putida
-
-
5.63