EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.65 | additional information | two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria | Pseudomonas putida |
1.2.1.65 | additional information | two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.65 | nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) | Pseudomonas putida |
1.2.1.65 | nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.65 | Al3+ | inhibits NahF activity by 42%; inhibits NahV activity by 15% | Pseudomonas putida | |
1.2.1.65 | Ba2+ | inhibits NahF activity by 62%; inhibits NahV activity by 52% | Pseudomonas putida | |
1.2.1.65 | Ca2+ | inhibits NahF activity by 63%; inhibits NahV activity by 35% | Pseudomonas putida | |
1.2.1.65 | Cs+ | inhibits NahF activity by 44%; inhibits NahV activity by 69% | Pseudomonas putida | |
1.2.1.65 | Cu2+ | inhibits NahF activity by 70% | Pseudomonas putida | |
1.2.1.65 | Li+ | inhibits NahF activity by 50%; inhibits NahV activity by 57% | Pseudomonas putida | |
1.2.1.65 | Mg2+ | inhibits NahF activity by 34%; inhibits NahV activity by 36% | Pseudomonas putida | |
1.2.1.65 | Ni2+ | inhibits NahF activity by 60%; inhibits NahV activity by 70% | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.65 | 0.00016 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.65 | 0.00109 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.65 | 0.034 | - |
Salicylaldehyde | - |
Pseudomonas putida | |
1.2.1.65 | 0.146 | - |
Salicylaldehyde | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.65 | Cu2+ | enhances NahV activity by 63% activity | Pseudomonas putida | |
1.2.1.65 | Fe2+ | enhances NahF activity by 40% activity | Pseudomonas putida | |
1.2.1.65 | Fe2+ | enhances NahV activity by 152% | Pseudomonas putida | |
1.2.1.65 | additional information | Zn2+ has no effect on NahF activity | Pseudomonas putida | |
1.2.1.65 | Zn2+ | enhances NahV activity by 14% activity | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.65 | 50000 | - |
1 * 50000, SDS-PAGE | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.65 | Pseudomonas putida | Q6XUJ4 | - |
- |
1.2.1.65 | Pseudomonas putida | Q6XUN9 | - |
- |
1.2.1.65 | Pseudomonas putida ND6 | Q6XUJ4 | - |
- |
1.2.1.65 | Pseudomonas putida ND6 | Q6XUN9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.65 | His-tagged NahF purified by Ni-NTA chromatography | Pseudomonas putida |
1.2.1.65 | His-tagged NahV purified by Ni-NTA chromatography | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.65 | 5-chlorosalicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 5-chlorosalicylate + NADH + H+ | - |
? | |
1.2.1.65 | 5-chlorosalicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | 5-chlorosalicylate + NADH + H+ | - |
? | |
1.2.1.65 | caprylic aldehyde + NAD+ + H2O | - |
Pseudomonas putida | caprylic acid + NADH + H+ | - |
? | |
1.2.1.65 | caprylic aldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | caprylic acid + NADH + H+ | - |
? | |
1.2.1.65 | formaldehyde + NAD+ + H2O | - |
Pseudomonas putida | formate + NADH + H+ | - |
? | |
1.2.1.65 | formaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | formate + NADH + H+ | - |
? | |
1.2.1.65 | glutaraldehyde + NAD+ + H2O | - |
Pseudomonas putida | glutarate + NADH + H+ | - |
? | |
1.2.1.65 | glutaraldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | glutarate + NADH + H+ | - |
? | |
1.2.1.65 | glyoxal + NAD+ + H2O | - |
Pseudomonas putida | glyoxylate + NADH + H+ | - |
? | |
1.2.1.65 | m-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | m-nitrobenzoate + NADH + H+ | - |
? | |
1.2.1.65 | o-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | o-methoxybenzoate + NADH + H+ | - |
? | |
1.2.1.65 | o-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida ND6 | o-methoxybenzoate + NADH + H+ | - |
? | |
1.2.1.65 | o-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | o-nitrobenzoate + NADH + H+ | - |
? | |
1.2.1.65 | salicylaldehyde + NAD+ + H2O | best substrate | Pseudomonas putida | salicylate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.65 | monomer | 1 * 50000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.65 | NahF | - |
Pseudomonas putida |
1.2.1.65 | NahV | - |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.65 | 50 | - |
72.8% activity is retained for NahF after a 30 min exposure | Pseudomonas putida |
1.2.1.65 | 50 | - |
NahV looses 88.8% activity after a 30 min exposure | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.65 | NAD+ | - |
Pseudomonas putida |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.2.1.65 | Pseudomonas putida | - |
- |
5.08 |
1.2.1.65 | Pseudomonas putida | - |
- |
5.63 |