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Literature summary extracted from

  • Liu, Z.; Pscheidt, B.; Avi, M.; Gaisberger, R.; Hartner, F.S.; Schuster, C.; Skranc, W.; Gruber, K.; Glieder, A.
    Laboratory evolved biocatalysts for stereoselective syntheses of substituted benzaldehyde cyanohydrins (2008), Chembiochem, 9, 58-61.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expressed in Pichia pastoris Prunus dulcis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.10 I108M/A111G mutant with improved catalytic properties: recovery of a 89.9% yield of (R)-2-chloromandelonitrile with an enantiomeric excess of 97.6% enantiomeric excess, about 4% residual aldehyde. The mutant also contains two silent mutations at position 85 and position 432 Prunus dulcis
4.1.2.10 L1Q mutation enhances expression in Pichia pastoris Prunus dulcis
4.1.2.10 L1Q/A111G activity is similar to wild-type enzyme with its preferred substrate benzaldehyde. Directed evolution of PaHNL5/L1Q/A11G Prunus dulcis
4.1.2.10 L1Q/N3I/I108M/A111G mutant with improved catalytic properties: recovery of a 92.7% yield of (R)-2-chloromandelonitrile with an ee of 98.6% enantiomeric excess, about 2% residual aldehyde, the mutant also contains two silent mutations at position 85 and position 432 Prunus dulcis
4.1.2.10 additional information directed evolution of PaHNL5/L1Q/A11G (a mutant with activity is similar to wild-type enzyme with its preferred substrate benzaldehyde). 10000 transformants are screened for cleavage or synthesis of (R)-2-chloromandelonitrile Prunus dulcis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.10 cyanide + benzaldehyde Prunus dulcis
-
 (R)-mandelonitrile
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus dulcis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + 2-chlorobenzaldehyde
-
 Prunus dulcis (R)-2-chloromandelonitrile
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Prunus dulcis (R)-mandelonitrile
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-HNL
-
 Prunus dulcis
4.1.2.10 HNL
-
 Prunus dulcis
4.1.2.10 Hydroxynitrile lyase
-
 Prunus dulcis
4.1.2.10 PaHNL5 isoenzyme 5 Prunus dulcis