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Literature summary extracted from

  • Kuppe, K.; Kerth, A.; Blume, A.; Ulbrich-Hofmann, R.
    Calcium-induced membrane microdomains trigger plant phospholipase D activity (2008), ChemBioChem, 9, 2853-2859.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate 500fold stimulation is observed upon incorporation of 10 mol 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate (POPA) into 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles in the presence of Ca2+ ions. Differential scanning calorimetry shows that the POPA-specific activation correlates with the phase behavior of the POPC/POPA vesicles in the presence of Ca2+ ions Brassica oleracea

Application

EC Number Application Comment Organism
3.1.4.4 molecular biology Ca2+-induced generation of membrane microdomains dramatically activates alpha-type phospholipase D from white cabbage Brassica oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 expressed in Escherichia coli Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ activity is Ca2+-dependent Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Brassica oleracea
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.4 -80°C, 10 mM Pipes buffer pH 7.0 Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Brassica oleracea 1,2-dihexanoyl-sn-glycero-3-phosphatidate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D
-
 Brassica oleracea
3.1.4.4 PLDalpha2
-
 Brassica oleracea