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Literature summary extracted from

  • You, D.; Xu, T.; Yao, F.; Zhou, X.; Deng, Z.
    Direct evidence that thil is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis (2008), ChemBioChem, 9, 1879-1882.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B5
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.B5 D189A mutation eliminates in vivo function of the enzyme Escherichia coli
2.7.7.B5 K321M mutation eliminates in vivo function of the enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.B5 0.24
-
 ATP pH 7.5, 22°C Escherichia coli
2.7.7.B5 0.84
-
 GTP pH 7.5, 22°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.B5 additional information Escherichia coli ThiI is involved in the modification of uridine to 4-thiouridine ?
-
 ?
2.7.7.B5 additional information Escherichia coli ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B5 additional information ThiI is involved in the modification of uridine to 4-thiouridine Escherichia coli ?
-
 ?
2.7.7.B5 additional information ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine Escherichia coli ?
-
 ?
2.7.7.B5 tRNA-uridine + ATP
-
 Escherichia coli adenylated-trNA-uridine + diphosphate
-
 ?
2.7.7.B5 tRNA-uridine + ATP based upon sequence alignments, ThiI shares a unique P-loop motif with the PPi synthetase family. ThiI very likely catalyzes the adenylation of a substrate, probably a molecule bearing a carbonyl oxygen Escherichia coli adenylated-trNA-uridine + diphosphate
-
 ?
2.7.7.B5 tRNA-uridine + GTP kcat/Km for GTP is about 6fold lower than the value for ATP Escherichia coli adenylated-trNA-uridine + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B5 ThiI
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.B5 22
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.B5 0.113
-
 GTP pH 7.5, 22°C Escherichia coli
2.7.7.B5 0.604
-
 ATP pH 7.5, 22°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.B5 7.5
-
 assay at Escherichia coli