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Literature summary extracted from

  • Wei, H.; Shi, L.; Wu, B.
    Production and characteristics of an enantioselective lipase from Burkholderia sp. GXU56 (2008), Chem. Eng. Technol., 31, 258-264.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 acetone 54% relative activity at 10% (v/v) acetone Burkholderia sp.
3.1.1.3 Co2+ 74% relative activity at 5 mM Burkholderia sp.
3.1.1.3 Cu2+ complete inhibition at 5 mM Burkholderia sp.
3.1.1.3 ethanol 56.2% relative activity at 10% (v/v) ethanol Burkholderia sp.
3.1.1.3 Fe2+ 36.9% relative activity at 5 mM Burkholderia sp.
3.1.1.3 Fe3+ 12.8% relative activity at 5 mM Burkholderia sp.
3.1.1.3 formaldehyde complete inhibition at 10% (v/v) Burkholderia sp.
3.1.1.3 Isopropanol 40.2% relative activity at 10% (v/v) isopropanol Burkholderia sp.
3.1.1.3 methanol 54.3% relative activity at 10% (v/v) methanol Burkholderia sp.
3.1.1.3 Mg2+ 96.6% relative activity at 5 mM Burkholderia sp.
3.1.1.3 Mn2+ 58.6% relative activity at 5 mM Burkholderia sp.
3.1.1.3 SDS 31.2% relative activity at 0.05% (w/v) SDS Burkholderia sp.
3.1.1.3 Zn2+ 28.9% relative activity at 5 mM Burkholderia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.038
-
 4-nitrophenyl butyrate at pH 8.0 and 40°C Burkholderia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ 107.2% realtive activity at 5 mM Burkholderia sp.
3.1.1.3 Li+ 106.5% relative activity at 5 mM Burkholderia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 35000
-
 SDS-PAGE Burkholderia sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 cetyltrimethylammonium bromidecetyltrimethylammonium bromide stimulated in the presence of 0.1% cetyltrimethylammonium bromidecetyltrimethylammonium bromide (145.3% relative activity) Burkholderia sp.
3.1.1.3 dimethylsulfoxide stimulated in the presence of 10% dimethylsulfoxide (105.1% relative activity) Burkholderia sp.
3.1.1.3 Triton X-100 stimulated in the presence of 0.1% Triton X-100 (110.2% relative activity) Burkholderia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Burkholderia sp.
-
-
-
3.1.1.3 Burkholderia sp. GXU56
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 Mono Q column chromatography, octyl-Sepharose column chromatography, and Sepharyl S-200 gel filtration Burkholderia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 61.42
-
 cell-free supernatant, at 40°C, pH 8.0 Burkholderia sp.
3.1.1.3 3294
-
 after 53.6fold purification, at 40°C, pH 8.0 Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Burkholderia sp. 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Burkholderia sp. GXU56 4-nitrophenol + butyrate
-
 ?
3.1.1.3 methyl (R)-mandelate + H2O
-
 Burkholderia sp. (R)-mandelic acid + methanol
-
 ?
3.1.1.3 methyl (R)-mandelate + H2O
-
 Burkholderia sp. GXU56 (R)-mandelic acid + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Burkholderia sp.
3.1.1.3 triacylglycerol acylhydrolase
-
 Burkholderia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Burkholderia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 45
-
 stable below 45°C Burkholderia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Burkholderia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 7 8.5 retains 96.3% of the initial activity after preservation for 2 h at 30°C and pH 8.0 Burkholderia sp.