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Literature summary extracted from

  • Geyer, B.C.; Woods, R.R.; Mor, T.S.
    Increased organophosphate scavenging in a butyrylcholinesterase mutant (2008), Chem. Biol. Interact., 175, 376-379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8 expression of a A328W/Y332A mutant BChE with improved cocaine hydrolase activity in transgenic Nicotiana benthamiana plants Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 A328W/Y332A the engineered recombinant cocaine hydrolase displays increased cocaine hydrolysis ability and increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 chlorpyrifos-oxon
-
 Homo sapiens
3.1.1.8 diisopropylfluorophosphate
-
 Homo sapiens
3.1.1.8 malaoxon
-
 Homo sapiens
3.1.1.8 additional information the engineered recombinant BChE mutant A328W/Y332A displays increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview Homo sapiens
3.1.1.8 neostigmine bromide
-
 Homo sapiens
3.1.1.8 paraoxon
-
 Homo sapiens
3.1.1.8 tetra(monoisopropyl) diphosphortetramide i.e. iso-OMPA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.042
-
 cocaine A328W/Y332A mutant BChE Homo sapiens
3.1.1.8 0.434
-
 cocaine wild-type BChE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.8 cocaine + H2O Homo sapiens
-
 ecgonine methyl ester + benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 cocaine + H2O
-
 Homo sapiens ecgonine methyl ester + benzoate
-
 ?
3.1.1.8 cocaine + H2O cv. EC 3.1.1.1 Homo sapiens ecgonine methyl ester + benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 BChE
-
 Homo sapiens
3.1.1.8 butyrylcholinesterase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 386.8
-
 cocaine A328W/Y332A mutant BChE Homo sapiens
3.1.1.8 418.3
-
 cocaine wild-type BChE Homo sapiens