EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | Triton X-100 | 2% nonionic surfactant of Triton X-100 activates by 51% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Tween 20 | 2% nonionic surfactant of Tween 20 activates by 38% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Tween 40 | 2% nonionic surfactant of Tween 40 activates by 6% at 25°C for 30 min | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.14 | Cu2+ | inhibits CHT1 | Pseudomonas sp. | |
3.2.1.14 | Fe2+ | complete inhibition of CHT1 | Pseudomonas sp. | |
3.2.1.14 | Mn2+ | complete inhibition of CHT1 | Pseudomonas sp. | |
3.2.1.14 | SDS | partial inhibition at 2 mM | Pseudomonas sp. | |
3.2.1.14 | Tween-20 | 21% inhibition at 2% | Pseudomonas sp. | |
3.2.1.132 | Cu2+ | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | EDTA | 10 mM inhibits by 86% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Fe2+ | 5 mM inhibits by 67% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Mn2+ | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | PMSF | 5 mM completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | SDS | 2 mM of the anionic surfactant SDS completely inhibits at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Triton X-100 | 0.5% nonionic surfactant of Triton X-100 inhibits by 56% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Tween 20 | 0.5% nonionic surfactant of Tween 20 inhibits by 16% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Tween 40 | 0.5% nonionic surfactant of Tween 40 inhibits by 29% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | Zn2+ | 5 mM inhibits by 21% at 25°C for 30 min | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | Ca2+ | 5 mM activates by 11% at 25°C for 30 min | Pseudomonas sp. | |
3.2.1.132 | additional information | 5 mM of Mg2+, Ba2+ have no effect on activity | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 68000 | - |
gel filtration | Pseudomonas sp. |
3.2.1.132 | 30000 | - |
x * 30000, SDSPAGE | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.14 | Pseudomonas sp. | - |
- |
- |
3.2.1.14 | Pseudomonas sp. TKU015 | - |
- |
- |
3.2.1.132 | Pseudomonas sp. | - |
- |
- |
3.2.1.132 | Pseudomonas sp. TKU015 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.14 | native enzyme 6fold from strain TKU015 by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Pseudomonas sp. |
3.2.1.132 | by gel filtration, to homogeneity, 6.4fold | Pseudomonas sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.14 | additional information | strain TKU015 grows with shrimp shell wastes as the sole carbon and nitrogen source | Pseudomonas sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 0.0069 | - |
purified CHT1, substrate chitin | Pseudomonas sp. |
3.2.1.14 | 0.0229 | - |
purified CHT1, substrate colloidal chitin | Pseudomonas sp. |
3.2.1.132 | 9.3 | - |
culture supernatant | Pseudomonas sp. |
3.2.1.132 | 59.1 | - |
6.4fold purified enzyme | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.14 | chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. | ? | - |
? | |
3.2.1.14 | chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. TKU015 | ? | - |
? | |
3.2.1.14 | colloidal chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. | ? | - |
? | |
3.2.1.14 | colloidal chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. TKU015 | ? | - |
? | |
3.2.1.132 | chitosan + H2O | activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan | Pseudomonas sp. | ? | - |
? | |
3.2.1.132 | chitosan + H2O | activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan | Pseudomonas sp. TKU015 | ? | - |
? | |
3.2.1.132 | additional information | no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan | Pseudomonas sp. | ? | - |
? | |
3.2.1.132 | additional information | no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan | Pseudomonas sp. TKU015 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.14 | monomer | 1 * 68000, CHT1, SDS-PAGE | Pseudomonas sp. |
3.2.1.132 | ? | x * 30000, SDSPAGE | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.14 | CHT1 | - |
Pseudomonas sp. |
3.2.1.132 | Chs1 | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 50 | - |
CHT1 | Pseudomonas sp. |
3.2.1.132 | 50 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 50 | - |
stable below, purified CHT1 | Pseudomonas sp. |
3.2.1.132 | 25 | 40 | maintains its initial activity, has almost half of its activity at 50°C but is completely inactivated at 70°C | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 6 | - |
CHT1 | Pseudomonas sp. |
3.2.1.132 | 4 | - |
- |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.14 | 5 | 7 | purified CHT1 | Pseudomonas sp. |
3.2.1.132 | 3 | 9 | - |
Pseudomonas sp. |