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Literature summary extracted from

  • Omokoko, B.; Jaentges, U.K.; Zimmermann, M.; Reiss, M.; Hartmeier, W.
    Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator (2008), BMC Microbiol., 8, 197.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.20 16900
-
 x * 44200, sequence analysis, monooxygenase subunit of the phenol hydroxylase (PheA1). x * 16900, sequence analysis, flavin reductase subunit of the phenol hydroxylase (PheA2) Geobacillus stearothermophilus
1.14.14.20 44200
-
 x * 44200, sequence analysis, monooxygenase subunit of the phenol hydroxylase (PheA1). x * 16900, sequence analysis, flavin reductase subunit of the phenol hydroxylase (PheA2) Geobacillus stearothermophilus
4.1.1.77 28900
-
 calculated from the deduced amino acid sequences Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.77 4-Oxalocrotonate Geobacillus stearothermophilus
-
 2-Oxopent-4-enoate + CO2
-
 ?
4.1.1.77 4-Oxalocrotonate Geobacillus stearothermophilus DSM 6285
-
 2-Oxopent-4-enoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.20 Geobacillus stearothermophilus
-
-
-
4.1.1.77 Geobacillus stearothermophilus B0VXM8 DSM6285
-
4.1.1.77 Geobacillus stearothermophilus DSM 6285 B0VXM8 DSM6285
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.20 phenol + NAD(P)H + H+ + O2
-
 Geobacillus stearothermophilus catechol + NAD(P)+ + H2O
-
 ?
4.1.1.77 4-Oxalocrotonate
-
 Geobacillus stearothermophilus 2-Oxopent-4-enoate + CO2
-
 ?
4.1.1.77 4-Oxalocrotonate
-
 Geobacillus stearothermophilus DSM 6285 2-Oxopent-4-enoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.20 ? x * 44200, sequence analysis, monooxygenase subunit of the phenol hydroxylase (PheA1). x * 16900, sequence analysis, flavin reductase subunit of the phenol hydroxylase (PheA2) Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.20 PheA1
-
 Geobacillus stearothermophilus
1.14.14.20 PheA2
-
 Geobacillus stearothermophilus
1.14.14.20 phenol hydroxylase
-
 Geobacillus stearothermophilus
4.1.1.77 4-oxalocrotonate decarboxylase
-
 Geobacillus stearothermophilus
4.1.1.77 4OD
-
 Geobacillus stearothermophilus

General Information

EC Number General Information Comment Organism
1.14.13.7 physiological function the enzyme is a component of the operon encoding the meta-pathway genes, it catalyzes the first step of the phenol degradative meta-pathway Geobacillus stearothermophilus